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Titolo:
Analysis of expressed sequence tags of retinal pigment epithelium: cystatin C is an abundant transcript
Autore:
Paraoan, L; Grierson, I; Maden, BEH;
Indirizzi:
Univ Liverpool, Sch Biol Sci, Liverpool L69 3BX, Merseyside, England Univ Liverpool Liverpool Merseyside England L69 3BX , Merseyside, England Univ Liverpool, Dept Med, Unit Expt Ophthalmol, Liverpool L69 3BX, Merseyside, England Univ Liverpool Liverpool Merseyside England L69 3BX , Merseyside, England
Titolo Testata:
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
fascicolo: 4, volume: 32, anno: 2000,
pagine: 417 - 426
SICI:
1357-2725(200004)32:4<417:AOESTO>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYSTEINE PROTEINASE-INHIBITORS; GENE-EXPRESSION; CDNA CLONES; COMPLEMENTARY-DNA; LIBRARY; DIVERSITY; SECRETION; PROFILE; TESTIS; BRAIN;
Keywords:
cystatin C; expressed sequence tags; gene expression; retinal pigment epithelium;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Paraoan, L Univ Liverpool, Sch Biol Sci, Liverpool L69 3BX, Merseyside, England Univ Liverpool Liverpool Merseyside England L69 3BX e, England
Citazione:
L. Paraoan et al., "Analysis of expressed sequence tags of retinal pigment epithelium: cystatin C is an abundant transcript", INT J BIO C, 32(4), 2000, pp. 417-426

Abstract

In order to identify genes that are expressed in the retinal pigment epithelium (RPE), randomly chosen clones of a cDNA library of cultured human foetal RPE cells were analyzed by sequencing. Of 164 informative expressed sequence tags (ESTs), 88 matched the sequences of 74 genes for proteins of known or presumed function. Approximately; a third of these represented genes with involvement in gene:protein expression, with a major subcategory concerned with protein turnover. In particular, the gene coding for precursor cystatin C was represented by 3 independent ESTs, and plaque hybridization estimated the frequency of cystatin C clones in the library to be 1.3%. Cystatin C mRNA in cultured RPE cells was confirmed by Northern blotting and by reverse transcription-polymerase chain reaction (RT-PCR) with identification of the cystatin C sequence as the product of the reaction. The survey also revealed 25 novel human sequences representing genes that are active in RPE, One of these was localized near a recently identified, new autosomal recessive retinitis pigmentosa locus. In conclusion, the findings specifically demonstrate the unexpected presence of cystatin C mRNA at fairly high abundance in cultured human RPE cells, and, more generally, serve as a model study establishing the usefulness of the EST approach for further characterizing the molecular basis of the activities of the RPE. (C) 2000 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 22:06:25