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Titolo:
UP-REGULATION OF GLIAL FIBRILLARY ACIDIC PROTEIN IN THE RETINA OF PRIMATE EYES WITH EXPERIMENTAL GLAUCOMA
Autore:
TANIHARA H; HANGAI M; SAWAGUCHI S; ABE H; KAGEYAMA M; NAKAZAWA F; SHIRASAWA E; HONDA Y;
Indirizzi:
KYOTO UNIV,GRAD SCH MED,DEPT OPHTHALMOL & VISUAL SCI,SAKYO KU,KAWAHARA CHO 54 KYOTO 60601 JAPAN NIIGATA UNIV,SCH MED,DEPT OPHTHALMOL NIIGATA JAPAN SANTEN PHARMACEUT CO LTD NARA JAPAN
Titolo Testata:
Archives of ophthalmology
fascicolo: 6, volume: 115, anno: 1997,
pagine: 752 - 756
SICI:
0003-9950(1997)115:6<752:UOGFAP>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
PIGMENT EPITHELIAL-CELLS; FACTOR GENE-EXPRESSION; MULLER CELLS; GROWTH-FACTOR; RADIAL GLIA; DETACHMENT; RAT; DEGENERATION; ASTROCYTES; APOPTOSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
H. Tanihara et al., "UP-REGULATION OF GLIAL FIBRILLARY ACIDIC PROTEIN IN THE RETINA OF PRIMATE EYES WITH EXPERIMENTAL GLAUCOMA", Archives of ophthalmology, 115(6), 1997, pp. 752-756

Abstract

Objective: To identify molecular mechanisms of retinal responses to intraocular pressure elevation in primate experimental glaucoma. Methods: An experimental glaucoma model was created by repeated laser trabeculophotocoagulation. After the preparation of complementary DNAs from extracted total RNAs in the retinas, polymerase chain reaction (PCR) experiments were performed for the following screening target genes: beta-tubulin beta(2) and beta(5) and glial fibrillary acidic protein (GFAP). To investigate the amplified sequences derived from the PCR experiments, sequencing, subcloning, and Southern blot analysis of PCR products were performed. In addition, an immunohistochemical analysis was performed in an attempt to show the distribution of the target gene products in the retinas. Results: A series of PCR experiments suggested upregulation of gene expression for GFAP but not for beta-tubulins. Sequencing of the PCR products and results of the Southern blot analysisshowed that the amplified sequences were derived mainly from the target gene, GFAP, and that increased expression of GFAP was found despitethe severity of glaucoma. Immunohistochemical studies also demonstrated increased expression of GFAP proteins in Muller cells and astrocytes in the retinas of primate eyes with experimental glaucoma. Conclusion: Our study showed up-regulation of GFAP at gene and protein levels, which suggests that glial components in the retina may contribute to the pathologic processes induced by elevated intraocular pressure.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 09:06:38