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Titolo:
Morphological and cytological characteristics of somatic hybrids of Nicotiana tabacum L-(+) N-megalosiphon Heurk. et Mull.
Autore:
Ilcheva, V; San, LH; Dimitrov, B; Zagorska, N;
Indirizzi:
Bulgarian Acad Sci, Inst Genet, BU-1113 Sofia, Bulgaria Bulgarian Acad Sci Sofia Bulgaria BU-1113 Genet, BU-1113 Sofia, Bulgaria SEITA, Tobacco Inst, F-24100 Bergerac, France SEITA Bergerac France F-24100 TA, Tobacco Inst, F-24100 Bergerac, France
Titolo Testata:
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
fascicolo: 1, volume: 36, anno: 2000,
pagine: 69 - 73
SICI:
1054-5476(200001/02)36:1<69:MACCOS>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSLOCATION EVENTS; INSITU HYBRIDIZATION; GAMMA-FUSION; PLUMBAGINIFOLIA; RESISTANCE; SYLVESTRIS; PLANTS;
Keywords:
protoplast fusion; tobacco; ploidy; flow cytometry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Ilcheva, V Bulgarian Acad Sci, Inst Genet, BU-1113 Sofia, Bulgaria Bulgarian Acad Sci Sofia Bulgaria BU-1113 113 Sofia, Bulgaria
Citazione:
V. Ilcheva et al., "Morphological and cytological characteristics of somatic hybrids of Nicotiana tabacum L-(+) N-megalosiphon Heurk. et Mull.", IN VITRO-PL, 36(1), 2000, pp. 69-73

Abstract

Somatic hybrid plants of Nicotiana tabacum (bar) (+) Nicotiana megalosiphon (npt II) were recovered after polyethylene glycol (PEG) mediated fusion. Hybrid calluses were selected on the basis of their dual resistance to bialaphos and kanamycin or UV inactivation of the donor species (Nicotiana megalosiphon) protoplasts. The hybrid nature of the individual clones obtained was confirmed by AFLP analysis. An array of plants were recovered includingself-fertile hybrid plants with N. tabacum or N. megalosiphon phenotype, self-sterile plants with N. tabacum habit, leaf and intermediate flower morphology, self-sterile plants with N. megalosiphon habit, abnormal leaves andintermediate flowers, and self-sterile plants of N. megalosiphon type withabnormal characters. Viable pollen was observed in hybrid plants from the third group. The hybrids possessed a nuclear DNA content near that of the diploid tobacco or N. megalosiphon, and also near that of the tetraploid genome size of N. megalosiphon. The results provide evidence for nonpreferential loss of one of the parental genomes and spontaneous asymmetrization of hybrid plants. The present study shows that by means of somatic hybridization a great genetic diversity in the hybrid clones can be achieved.

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Documento generato il 04/07/20 alle ore 04:34:54