Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Protein kinase C independent activation of inducible nitric oxide synthaseby tumor necrosis factor-alpha in TM4 Sertoli cells
Autore:
Chung, CK; Chung, BH; Shin, T; Chae, HJ; Kim, HR; Cho, CC; Hong, GY; An, NH; Kim, HM;
Indirizzi:
Wonkwang Univ, Coll Pharm, Iksan 570749, Chonbuk, South Korea Wonkwang Univ Iksan Chonbuk South Korea 570749 0749, Chonbuk, South Korea Hallym Univ, Dept Food & Nutr, Chunchon 200702, South Korea Hallym Univ Chunchon South Korea 200702 tr, Chunchon 200702, South Korea Wonkwang Univ, Ctr Oriental Med Sci, Iksan 570749, Chonbuk, South Korea Wonkwang Univ Iksan Chonbuk South Korea 570749 0749, Chonbuk, South Korea Cheju Natl Univ, Coll Agr, Cheju, South Korea Cheju Natl Univ Cheju South Korea tl Univ, Coll Agr, Cheju, South Korea Wonkwang Univ, Sch Dent, Dept Dent Pharmacol, Iksan 570749, Chonbuk, SouthKorea Wonkwang Univ Iksan Chonbuk South Korea 570749 70749, Chonbuk, SouthKorea Wonkwang Univ, Sch Med, Dept Internal Med, Iksan 570749, Chonbuk, South Korea Wonkwang Univ Iksan Chonbuk South Korea 570749 0749, Chonbuk, South Korea Wonkwang Univ, Sch Med, Dept Obstet & Gynecol, Iksan 570749, Chonbuk, South Korea Wonkwang Univ Iksan Chonbuk South Korea 570749 0749, Chonbuk, South Korea
Titolo Testata:
IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY
fascicolo: 1, volume: 22, anno: 2000,
pagine: 49 - 59
SICI:
0892-3973(2000)22:1<49:PKCIAO>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
MACROPHAGES; INHIBITION; CYTOKINES; NITRATE;
Keywords:
nitric oxide; TM4 Sertoli cells; tumor necrosis factor-alpha; interferon-gamma; protein kinase C;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Kim, HM Wonkwang Univ, Coll Pharm, Iksan 570749, Chonbuk, South Korea Wonkwang Univ Iksan Chonbuk South Korea 570749 onbuk, South Korea
Citazione:
C.K. Chung et al., "Protein kinase C independent activation of inducible nitric oxide synthaseby tumor necrosis factor-alpha in TM4 Sertoli cells", IMMUNOPH IM, 22(1), 2000, pp. 49-59

Abstract

To investigate the nitric oxide (NO) production and its signalling mechanism in TM4 Sertoli cells, the cells were treated with recombinant tumor necrosis factor-alpha (rTNF-alpha), recombinant interleukin-1 alpha (rIL-1 alpha), or lipopolysaccharide (LPS), either alone or in combination with recombinant interferon-gamma (rIFN-gamma), and NO production was measured by using the Griess method. TM4 Sertoli cells produced a small amount of NO upon treatment with rIFN-gamma. The effect of rIFN-gamma was drastically increased by cotreatment with rTNF-alpha in a dose-dependent manner. However, combination of rIL-1 alpha or LPS with rIFN-gamma did not synergize to activate cells. RIFN-gamma in combination with rTNF-alpha showed marked increase of the expression of iNOS protein. Protein kinase C inhibitors did not inhibitthe production of NO induced by rIFN-gamma plus rTNF-alpha. These results suggest that the role of TNF-alpha is to provide TM4 Sertoli cells with theactive cofactor for NO production and TNF-alpha-induced signaling for induction of NO synthesis is not dependent on protein kinase C activation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 07:05:13