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Titolo:
Monoclonal antibody probes for p21(WAF1/CIP1) and the INK4 family of cyclin-dependent kinase inhibitors
Autore:
Thullberg, M; Welcker, M; Bartkova, J; Kjerulff, AA; Lukas, J; Hogberg, J; Bartek, J;
Indirizzi:
Danish Canc Soc, Inst Canc Biol, DK-2100 Copenhagen, Denmark Danish Canc Soc Copenhagen Denmark DK-2100 , DK-2100 Copenhagen, Denmark Karolinska Inst, Natl Inst Environm Med, S-17177 Stockholm, Sweden Karolinska Inst Stockholm Sweden S-17177 Med, S-17177 Stockholm, Sweden Natl Inst Working Life, S-17184 Solna, Sweden Natl Inst Working Life Solna Sweden S-17184 Life, S-17184 Solna, Sweden
Titolo Testata:
HYBRIDOMA
fascicolo: 1, volume: 19, anno: 2000,
pagine: 63 - 72
SICI:
0272-457X(200002)19:1<63:MAPFPA>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
RETINOBLASTOMA-PROTEIN; TUMOR SUPPRESSION; CDK6 INHIBITOR; BREAST-CANCER; GENE; EXPRESSION; DIFFERENTIATION; P27(KIP1); CELLS; P18;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Bartek, J Danish Canc Soc, Inst Canc Biol, Strandboulevarden 49, DK-2100 Copenhagen,Denmark Danish Canc Soc Strandboulevarden 49 Copenhagen Denmark DK-2100
Citazione:
M. Thullberg et al., "Monoclonal antibody probes for p21(WAF1/CIP1) and the INK4 family of cyclin-dependent kinase inhibitors", HYBRIDOMA, 19(1), 2000, pp. 63-72

Abstract

Inhibition of cyclin dependent kinases (cdk) by proteins of two families of cdk inhibitors (CKIs) represents one of the key modes of cell-cycle control. Although not fully understood at present, the functions of the individual members of the Cip/Kip and INK4 families of CKIs have been implicated infundamental biological processes as diverse as cellular proliferation, responses to genotoxic stress, regulation of cellular differentiation, and senescence. In addition, the seven currently known CKIs qualify as either established or candidate tumor suppressors whose loss or inactivation contribute to molecular pathogenesis of a wide range of tumor types. In this study, we report the isolation and characterization of a panel of 10 mouse monoclonal antibodies (MAbs) that specifically recognize p21(WAF1/CIP1) (p21) or the individual members of the INK4 family of CKIs: p15(INK4b) (p15), p16(INK4a) (p16), p18(INK4c) (p18), or p19(INK4d) (p19). These antibodies are proving to be invaluable molecular probes for analyses of protein abundance, subcellular localization, interacting cellular proteins, and ultimately the function(s) of these cell cycle regulators. Epitopes targeted by the antibodies were mapped by peptide enzyme-linked immunoadsorbent assay (ELISA), andperformance of the MAbs assessed in a range of immunochemical techniques. Individual MAbs of our series recognize distinct pools of the respective CKIs, a feature reflected by their differential applicability in immunoblotting, immunoprecipitation, and immunostaining including immunohistochemistry on archival paraffin-embedded tissue sections. Together, these antibodies represent useful reagents to study CKIs in cells and tissues, a set of toolsthat should help elucidate the physiological roles played by the individual CKIs, and better understand the molecular mechanisms of loss or inactivation of these (candidate) tumor suppressors in human malignancies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 21:54:40