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Titolo:
Simultaneous molecular karyotyping and mapping of viral DNA integration sites by 25-color COBRA-FISH
Autore:
Szuhai, K; Bezrookove, V; Wiegant, J; Vrolijk, J; Dirks, RW; Rosenberg, C; Raap, AK; Tanke, HJ;
Indirizzi:
Leiden State Univ, Med Ctr, Dept Mol Cell Biol, Lab Cytochem & Cytometry, NL-2333 AL Leiden, Netherlands Leiden State Univ Leiden Netherlands NL-2333 AL 3 AL Leiden, Netherlands
Titolo Testata:
GENES CHROMOSOMES & CANCER
fascicolo: 1, volume: 28, anno: 2000,
pagine: 92 - 97
SICI:
1045-2257(200005)28:1<92:SMKAMO>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
FLUORESCENCE INSITU HYBRIDIZATION; DIGITAL IMAGING MICROSCOPY; FRAGILE SITES; HUMAN PAPILLOMAVIRUS; HUMAN-CHROMOSOMES; CERVICAL-CANCER; MULTIPLE; PROBES; GENE; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Tanke, HJ Leiden State Univ, Med Ctr, Dept Mol Cell Biol, Lab Cytochem & Cytometry, Wassenaarseweg 72, NL-2333 AL Leiden, Netherlands Leiden State Univ Wassenaarseweg 72 Leiden Netherlands NL-2333 AL
Citazione:
K. Szuhai et al., "Simultaneous molecular karyotyping and mapping of viral DNA integration sites by 25-color COBRA-FISH", GENE CHROM, 28(1), 2000, pp. 92-97

Abstract

Combined binary ratio labeling (COBRA) fluorescence in situ hybridization (FISH) allows 24-color FISH karyotyping of human metaphase chromosomes utilizing only four fluorochromes, instead of the five required for combinatorial labeling procedures. Here we show that by introduction of a fifth fluorochrome, COBRA-FISH permits molecular cytogenetic mapping of viral integration sites in complex karyotypes in the context of a 24-color hybridization. We were able to detect a single copy of the human papillomavirus 16 in the SiHa cell line and to confirm the site of integration at 13q21-31. We also demonstrate the gene mapping possibility of 25-color hybridization by detecting a MYC cosmid on normal metaphase chromosomes. the possibility of mapping single-copy probes in the background of 24-color hybridization expands the tools for cytogenetic mapping of DNA sequences and will contribute to the understanding of the role of viral integration and chromosome rearrangement in virus-mediated carcinogenesis. Genes Chromosomes Cancer 28:92-97, 2000. (C) 2000 WiIey-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 14:48:28