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Titolo:
Identification of the regions involved in DNA binding by the mouse PEBP2 alpha protein
Autore:
Perez-Alvarado, GC; Munnerlyn, A; Dyson, HJ; Grosschedl, R; Wright, PE;
Indirizzi:
Scripps Clin & Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA Scripps Clin & Res Inst La Jolla CA USA 92037 iol, La Jolla, CA 92037 USA Scripps Clin & Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA Scripps Clin & Res Inst La Jolla CA USA 92037 iol, La Jolla, CA 92037 USA Univ Calif San Francisco, Howard Hughes Med Inst, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Univ Calif San Francisco, Dept Microbiol, San Francisco, CA 94143 USA UnivCalif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Univ Calif San Francisco, Dept Biochem, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Univ Munich, Ctr Gene, D-81377 Munich, Germany Univ Munich Munich Germany D-81377 ch, Ctr Gene, D-81377 Munich, Germany Univ Munich, Inst Biochem, D-81377 Munich, Germany Univ Munich Munich Germany D-81377 Inst Biochem, D-81377 Munich, Germany
Titolo Testata:
FEBS LETTERS
fascicolo: 2, volume: 470, anno: 2000,
pagine: 125 - 130
SICI:
0014-5793(20000324)470:2<125:IOTRII>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSCRIPTION FACTOR PEBP2; ZINC SUPEROXIDE-DISMUTASE; LEUKEMIA-VIRUS ENHANCERS; RUNT HOMOLOGY DOMAIN; CLEIDOCRANIAL DYSPLASIA; BACKBONE ASSIGNMENT; CRYSTAL-STRUCTURE; CHEMICAL-SHIFTS; AML1 GENE; NMR;
Keywords:
polyoma virus enhancer binding protein 2 alpha; runt domain; specific DNA binding; conformational change; nuclear magnetic resonance spectroscopy; chemical shift deviation mapping;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Wright, PE Scripps Clin & Res Inst, Dept Mol Biol, 10550 N Torrey Pines Rd, La Jolla,CA 92037 USA Scripps Clin & Res Inst 10550 N Torrey Pines Rd La Jolla CA USA 92037
Citazione:
G.C. Perez-Alvarado et al., "Identification of the regions involved in DNA binding by the mouse PEBP2 alpha protein", FEBS LETTER, 470(2), 2000, pp. 125-130

Abstract

The polyomavirus enhancer binding protein 2 alpha (PEBP2 alpha) is a DNA binding transcriptional regulatory protein that binds conserved sites in thepolyomavirus enhancer, mammalian type C retroviral enhancers and T-cell receptor gene enhancers. Binding of PEBP2 alpha and homologous proteins to the consensus DNA sequence TGPyGGTPy is mediated through a protein domain known as the runt domain. Although recent NMR studies of DNA-bound forms of the runt domain have shown an immunoglobulin-like (Ig) fold, the identification of residues of the protein that are involved in DNA binding has been obscured by the low solubility of the runt domain, Constructs of the mouse PEBP2 alpha A1 gene were generated with N- and C-terminal extensions beyond the runt homology region, The construct containing residues Asp90 to Lys225 of the sequence (PEBP2 alpha 90-225) yielded soluble protein. The residues that participate in DNA binding were determined by comparing the NMR spectraof free and DNA-bound PEBP2 alpha 90-225, Analysis of the changes in the NMR spectra of the two forms of the protein by chemical shift deviation mapping allowed the unambiguous determination of the regions that are responsible for specific DNA recognition by PEBP2 alpha. Five regions in PEBP2 alpha90-225 that are localized at one end of the beta-barrel were found to interact with DNA, similar to the DNA binding interactions of other Ig fold proteins. (C) 2000 Federation of European Biochemical Societies.

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Documento generato il 04/04/20 alle ore 14:51:09