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Titolo:
Spontaneous keratinocyte cell lines representing early and advanced stagesof malignant transformation of the epidermis
Autore:
Proby, CM; Purdie, KJ; Sexton, CJ; Purkis, P; Navsaria, HA; Stables, JN; Leigh, IM;
Indirizzi:
Univ London Queen Mary & Westfield Coll, St Bartholomews & Royal London Sch Med & Dent, Ctr Cutaneous Res, Dept Acad Dermatol, London E1 2AT, EnglandUniv London Queen Mary & Westfield Coll London England E1 2AT T, England Wellcome Res Labs, Dept Cell Biol, Beckenham BR3 3BS, Kent, England Wellcome Res Labs Beckenham Kent England BR3 3BS m BR3 3BS, Kent, England
Titolo Testata:
EXPERIMENTAL DERMATOLOGY
fascicolo: 2, volume: 9, anno: 2000,
pagine: 104 - 117
SICI:
0906-6705(200004)9:2<104:SKCLRE>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
RENAL-TRANSPLANT RECIPIENTS; NONMELANOMA SKIN CANCERS; HUMAN-PAPILLOMAVIRUS DNA; EPIDERMODYSPLASIA VERRUCIFORMIS; MONOCLONAL-ANTIBODIES; ALLOGRAFT RECIPIENTS; TISSUE DISTRIBUTION; COMPLEX EPITHELIA; HIGH-FREQUENCY; BREAST-CANCER;
Keywords:
non-melanoma skin cancer; squamous cell carcinoma; epithelial keratins; human papillomavirus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Proby, CM Univ London Queen Mary & Westfield Coll, St Bartholomews & RoyalLondon Sch Med & Dent, Ctr Cutaneous Res, Dept Acad Dermatol, Clin Sci ResBldg,2 Newark St, London E1 2AT, England Univ London Queen Mary & Westfield Coll Clin Sci Res Bldg,2 Newark St London England E1 2AT
Citazione:
C.M. Proby et al., "Spontaneous keratinocyte cell lines representing early and advanced stagesof malignant transformation of the epidermis", EXP DERMATO, 9(2), 2000, pp. 104-117

Abstract

A unique series of epidermal cell lines representing different stages of malignant transformation were spontaneously derived from a single adult immunosuppressed individual. Four keratinocyte lines (PM1-4) established from forehead skin are here compared with 4 squamous cell carcinoma (SCC) lines (MET1-4) derived respectively from a primary cutaneous tumour, two local recurrences and a distant metastasis of invasive SCC. Despite altered growth properties, the PM lines retained many features of normal keratinocytes including keratin phenotype, differentiation capacity and non-tumorigenicity inathymic mice. In contrast, from early passage, the MET lines displayed markedly reduced growth requirements, abnormal differentiation, aberrant K18 expression and tumorigenicity in athymic mice. The abnormal keratin profile of individual MET lines closely reflected the keratin phenotype of the tumour of origin. Although unusual HPV types were identified in the original tissue, there was no evidence of persistent virus within any cell line and itappears that HPV is not critical for maintenance of the immortal phenotype. The PM lines were distinctly different from invasive SCC lines and are likely to be useful for studies of mutations important early in neoplastic progression. The SCC series represent primary, recurrent and metastatic carcinoma. Availability of such a series from the same individual will facilitate genetic analysis of the malignant process.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 14:24:44