Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Detection and quantification of Spongospora subterranea f. sp subterranea in soils and on tubers using specific PCR primers
Autore:
Bell, KS; Roberts, J; Verrall, S; Cullen, DW; Williams, NA; Harrison, JG; Toth, IK; Cooke, DEL; Duncan, JM; Claxton, JR;
Indirizzi:
Scottish Crop Res Inst, Dept Fungal & Bacterial Plant Pathol, Dundee DD2 5DA, Scotland Scottish Crop Res Inst Dundee Scotland DD2 5DA Dundee DD2 5DA, Scotland
Titolo Testata:
EUROPEAN JOURNAL OF PLANT PATHOLOGY
fascicolo: 9, volume: 105, anno: 1999,
pagine: 905 - 915
SICI:
0929-1873(199912)105:9<905:DAQOSS>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMODIOPHORA-BRASSICAE; RIBOSOMAL DNA; SPORE BALLS; POTATO; SEQUENCES; FUNGI; GENE; RNA;
Keywords:
cystosori; internal transcribed spacer regions; plasmodiophorids; potato; powdery scab; soil;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Duncan, JM Scottish Crop Res Inst, Dept Fungal & Bacterial Plant Pathol, Dundee DD2 5DA, Scotland Scottish Crop Res Inst Dundee Scotland DD2 5DA 5DA, Scotland
Citazione:
K.S. Bell et al., "Detection and quantification of Spongospora subterranea f. sp subterranea in soils and on tubers using specific PCR primers", EUR J PL P, 105(9), 1999, pp. 905-915

Abstract

PCR-based methods were developed for the detection and quantification of the potato pathogen Spongospora subterranea f. sp. subterranea (S. subterranea) in peel, tuber washings and soil. A partial sequence was obtained for S. subterranea ribosomal DNA and specific PCR primers (Sps1 and Sps2) were chosen from the internal transcribed spacer regions. These primers amplifieda 391 bp product from S. subterranea DNA but did not amplify DNA from potato or a range of soil-borne microbes, including related species. Diluted S.subterranea DNA was detected at a concentration equivalent to 25 x 10(-5) cystosori or 1 zoospore per PCR. Amplification was detected from peel and washings of infected and apparently healthy tubers, but not from peel of Scottish classified seed potatoes or axenically micropropagated potatoes. A rapid method for extracting S. subterranea DNA from soils was developed. Thisyielded DNA pure enough for PCR within 3 h and facilitated the detection of 1-5 cystosori per gram of soil. A PCR quantification technique was developed involving comparison of product ratios obtained after co-amplification of S. subterranea DNA along with an internal standard (competitor DNA fragment). This quantitative technique was also adapted for use in soil. PCR detection of S. subterranea in soil was considerably more sensitive than previously reported immunoassays and was quicker and easier than conventional bait plant bioassays. Such an assay could be useful for developing disease risk assessments for field soils and seed potato stocks and for future studies on the ecology and control of S. subterranea.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 05:26:55