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Titolo:
Fathead minnow vitellogenin: Complementary DNA sequence and messenger RNA and protein expression after 17 beta-estradiol treatment
Autore:
Korte, JJ; Kahl, MD; Jensen, KM; Pasha, MS; Parks, LG; LeBlanc, GA; Ankley, GT;
Indirizzi:
US EPA, Mid Continent Ecol Div, Duluth, MN 55804 USA US EPA Duluth MN USA55804 , Mid Continent Ecol Div, Duluth, MN 55804 USA N Carolina State Univ, Dept Toxicol, Raleigh, NC 27695 USA N Carolina State Univ Raleigh NC USA 27695 Toxicol, Raleigh, NC 27695 USA
Titolo Testata:
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
fascicolo: 4, volume: 19, anno: 2000,
parte:, 1
pagine: 972 - 981
SICI:
0730-7268(200004)19:4<972:FMVCDS>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
TROUT ONCORHYNCHUS-MYKISS; RAINBOW-TROUT; GENE-EXPRESSION; POSTTRANSCRIPTIONAL REGULATION; ESTROGENIC CHEMICALS; ESTRADIOL; BIOMARKER; RECEPTOR; FISH; NONYLPHENOL;
Keywords:
vitellogenin; Pimephales promelas; 17 beta-estradiol; mRNA; cDNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Korte, JJ US EPA, Mid Continent Ecol Div, 6201 Congdon Blvd, Duluth, MN 55804 USA US EPA 6201 Congdon Blvd Duluth MN USA 55804 uluth, MN 55804 USA
Citazione:
J.J. Korte et al., "Fathead minnow vitellogenin: Complementary DNA sequence and messenger RNA and protein expression after 17 beta-estradiol treatment", ENV TOX CH, 19(4), 2000, pp. 972-981

Abstract

Induction of vitellogenin (VTG) in oviparous animals has been proposed as a sensitive indicator of environmental contaminants that activate the estrogen receptor. In the present study, a sensitive ribonuclease protection assay (RPA) for VTG messenger RNA (mRNA) was developed for the fathead minnow (Pimephales promelas), a species proposed for routine endocrine-disrupting chemical (EDC) screening. The utility of this method was compared with an enzyme-linked immunosorbent assay (ELISA) specific for fathead minnow VTG protein. Assessment of the two methods included kinetic characterization of the plasma VTG protein and hepatic VTG mRNA levels in male fathead minnows following intraperitoneal injections of 17 beta-estradiol (E2) at two dose levels (0.5. 5.0 mg/kg). Initial plasma E2 concentrations were elevated in adose-dependent manner but returned to normal levels within 2 d. Liver VTG mRNA was detected within 4 h, reached a maximum around 48 h, and returned to normal levels in about 6 d. Plasma VTG protein was detectable within 16 hof treatment, reached maximum levels at about 72 h, and remained near these maximum levels for at least 18 d. While the RPA was about 1,000 times more sensitive than the ELISA, the ELISA appears superior for routine screening tests. The ELISA method is relatively simple to perform and, because males lack a clearance mechanism for VTG, the protein remains at relatively high concentrations in the plasma for an extended period of time. As part of the development of the RPA, the complementary DNA (cDNA) sequence for fathead minnow VTG was determined and the deduced amino acid sequence compared with VTG sequences for other fish species.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 23:49:34