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Titolo:
Eicosanoid regulation of angiogenesis: role of endothelial arachidonate 12-lipoxygenase
Autore:
Nie, DT; Tang, KQ; Diglio, C; Honn, KV;
Indirizzi:
Wayne State Univ, Sch Med, Dept Radiat Oncol, Detroit, MI 48202 USA Wayne State Univ Detroit MI USA 48202 Radiat Oncol, Detroit, MI 48202 USA Wayne State Univ, Sch Med, Dept Pathol, Detroit, MI 48201 USA Wayne State Univ Detroit MI USA 48201 Dept Pathol, Detroit, MI 48201 USA Karmanos Canc Inst, Detroit, MI USA Karmanos Canc Inst Detroit MI USAKarmanos Canc Inst, Detroit, MI USA
Titolo Testata:
BLOOD
fascicolo: 7, volume: 95, anno: 2000,
pagine: 2304 - 2311
SICI:
0006-4971(20000401)95:7<2304:EROARO>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIBROBLAST GROWTH-FACTOR; TUMOR-CELL ADHESION; PROTEIN-KINASE; 12(S)-HYDROXYEICOSATETRAENOIC ACID; INTEGRIN ALPHA(V)BETA(3); PLATELET 12-LIPOXYGENASE; ALPHA-V-BETA-3 INTEGRINS; SURFACE EXPRESSION; UP-REGULATION; 12(S)-HETE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Honn, KV Wayne State Univ, Sch Med, Dept Radiat Oncol, 431 Chem Bldg, Detroit, MI 48202 USA Wayne State Univ 431 Chem Bldg Detroit MI USA 48202 MI 48202 USA
Citazione:
D.T. Nie et al., "Eicosanoid regulation of angiogenesis: role of endothelial arachidonate 12-lipoxygenase", BLOOD, 95(7), 2000, pp. 2304-2311

Abstract

Angiogenesis, the formation of new capillaries from preexisting blood vessels, is a multistep, highly orchestrated process involving vessel sprouting, endothelial cell migration, proliferation, tube differentiation, and survival. Eicosanoids, arachidonic acid (AA)-derived metabolites, have potent biologic activities on vascular endothelial cells. Endothelial cells can synthesize various eicosanoids, including the 12-lipoxygenase (LOX) product 12(S)-hydroxyeicosatetraenoic acid (HETE), Here we demonstrate that endogenous 12-LOX is involved in endothelial cell angiogenic responses. First, the 12-LOX inhibitor, N-benzyl-N-hydroxy-5-phenylpentanamide (BHPP), reduced endothelial cell proliferation stimulated either by basic fibroblast growth factor (bFGF) or by vascular endothelial growth factor (VEGF), Second, 12-LOXinhibitors blocked VEGF-induced endothelial cell migration, and this blockage could be partially reversed by the addition of 12(S)-HETE. Third, pretreatment of an angiogenic endothelial cell line, RV-ECT, with BHPP significantly inhibited the formation of tubelike/cordlike structures within Matrigel. Fourth, overexpression of 12-LOX in the CD4 endothelial cell line significantly stimulated cell migration and tube differentiation. In agreement with the critical role of 12-LOX in endothelial cell angiogenic responses in vitro, the 12-LOX inhibitor BHPP significantly reduced bFGF-induced angiogenesis in vivo using a Matrigel implantation bioassay, These findings demonstrate that AA metabolism in endothelial cells, especially the 12-LOX pathway, plays a critical role in angiogenesis.(Blood .2000;95:2304-2311) (C) 2000 by The American Society of Hematology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 11:57:10