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Titolo:
High resolution detection of rRNA and rDNA in plant nucleoli with different activities by in situ hybridization
Autore:
Bassy, O; Jimenez-Garcia, LF; Echeverria, OM; Vazquez-Nin, GH; de la Espina, SMD;
Indirizzi:
CSIC, Ctr Invest Biol, Dpto Biol Vegetal, Lab Matriz Nucl, E-28006 Madrid,Spain CSIC Madrid Spain E-28006 Vegetal, Lab Matriz Nucl, E-28006 Madrid,Spain UNAM, Fac Ciencias, Dpto Biol, Lab Microscopia Elect, Mexico City, DF, Mexico UNAM Mexico City DF Mexico ab Microscopia Elect, Mexico City, DF, Mexico
Titolo Testata:
BIOLOGY OF THE CELL
fascicolo: 1, volume: 92, anno: 2000,
pagine: 59 - 70
SICI:
0248-4900(200001)92:1<59:HRDORA>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
RIBOSOMAL-RNA GENES; DIFFERENT PATTERNS; FIBRILLAR CENTERS; PISUM-SATIVUM; CELL-CYCLE; HELA-CELLS; TRANSCRIPTION; ORGANIZATION; ULTRASTRUCTURE; AUTORADIOGRAPHY;
Keywords:
plant cells; nucleolus; rRNA; rDNA; ultrastructure;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: de la Espina, SMD CSIC, Ctr Invest Biol, Dpto Biol Vegetal, Lab Matriz Nucl, Velazquez 144, E-28006 Madrid, Spain CSIC Velazquez 144 Madrid Spain E-28006 6 Madrid, Spain
Citazione:
O. Bassy et al., "High resolution detection of rRNA and rDNA in plant nucleoli with different activities by in situ hybridization", BIO CELL, 92(1), 2000, pp. 59-70

Abstract

Ln the present work we perform in situ hybridization with probes to different stretches of rDNA and electron microscopy of nucleoli with different activities, to gain insight into the ultrastructural organization of transcription and processing in the plant nucleolus. The main ultrastructural nucleolar components: fibrillar centers (FC), dense fibrillar component (DFC), and granular component (GC), are arranged in different ways depending on nucleolar activity. Heterogeneous FCs containing RNP fibrils and nucleolar perichromatin granules are frequently seen in nucleoli in the process of activation. DNA-RNA in situ hybridization with biotinylated probes spanning different sequences of the rDNA unit followed by immunogold detection of biotin, demonstrated the localization of the ribosomal transcripts in DFC, mainlyin the zones around the FCs, in GC, and in the periphery of pale FC. The internal region of the heterogeneous FCs is labeled only in cells in the process of activation of transcription after dormancy. The distribution of theU3 probe indicates that the processing of the rRNA takes place in the DFC and inside the heterogeneous FCs, in which transcription occurs. DNA-DNA hybridization demonstrates the presence of rDNA in the compact and extended chromatin located in the interior and at the periphery of I;Cs and in nucleolar associated chromatin. Our results support the view that the plant nucleolus has a highly dynamic morphological and functional organization composed of a bipartite domain formed by FCs surrounded by DFC, which is associated with rRNA transcription and processing, and the GC representing a store of preribosomal particles. 2000 (C) Editions scientifiques et medicales Elsevier SAS.

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Documento generato il 04/12/20 alle ore 06:37:20