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Titolo:
A specific inhibitor of p34(cdc2)/cyclin B suppresses fertilization-induced calcium oscillations in mouse eggs
Autore:
Deng, MQ; Sheldon, SS;
Indirizzi:
Iowa State Univ Sci & Technol, Dept Zool & Genet, Ames, IA 50011 USA Iowa State Univ Sci & Technol Ames IA USA 50011 Genet, Ames, IA 50011 USA
Titolo Testata:
BIOLOGY OF REPRODUCTION
fascicolo: 4, volume: 62, anno: 2000,
pagine: 873 - 878
SICI:
0006-3363(200004)62:4<873:ASIOPB>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
MATURATION-PROMOTING FACTOR; CYCLIN-DEPENDENT KINASES; INDUCED CA2+ OSCILLATIONS; METAPHASE-II ARREST; MAMMALIAN EGGS; PROTEIN-KINASE; OOCYTES; RELEASE; ROSCOVITINE; TRANSIENTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Deng, MQ Univ Connecticut, Dept Anim Sci, Biotech Ctr, 3636 Horsebarn Rd,Extens U-40, Storrs, CT 06269 USA Univ Connecticut 3636 Horsebarn Rd,Extens U-40 Storrs CT USA 06269
Citazione:
M.Q. Deng e S.S. Sheldon, "A specific inhibitor of p34(cdc2)/cyclin B suppresses fertilization-induced calcium oscillations in mouse eggs", BIOL REPROD, 62(4), 2000, pp. 873-878

Abstract

Fertilization-induced Ca2+ oscillations in mouse eggs cease at the time ofpronuclear formation when maturation-promoting factor (MPF) is inactivated, but the Ca2+ oscillations are ceaseless if eggs are arrested at metaphaseby colcemid, which maintains the activity of MPF. To determine the possible role of MPF in regulation of cytoplasmic Ca2+ excitability, roscovitine, a specific inhibitor of p34(cdc2)/cyclin B kinase, was used to inactivate MPF, and its effect on fertilization-induced Ca2+ oscillations was investigated. Our results showed that roscovitine at greater than or equal to 50 mu M suppressed fertilization-induced Ca2+ oscillations in normal and colcemid-treated metaphase II (MII) eggs after the first 1-2 Ca2+ spikes. Roscovitine inhibition of fertilization-induced Ca2+ oscillations could be reversed by extensive washing of the eggs. Histone H1 kinase activity in colcemid-treated MII eggs was similarly inhibited by roscovitine, which suggested thatthe cessation of fertilization-induced Ca2+ oscillations is due to the inactivation of MPF. Thimerosal-induced Ca2+ oscillations in Ca2+-, Mg2+-free medium was also suppressed by roscovitine, suggesting a general inhibitory effect of roscovitine on Ca2+ oscillations. The inhibition may be achieved by disruption of Ca2+ release and refilling of the calcium store. Thapsigargin, an inhibitor of the endoplasmic reticulum Ca-ATPase, induced significantly less Ca2+ release in roscovitine-treated eggs than in the non-drug-treated eggs. Taken together, our results suggest that MPF plays an important role in regulation of the cytoplasmic Ca2+ excitability in mouse eggs.

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Documento generato il 24/11/20 alle ore 08:32:00