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Titolo:
Mechanisms of nonimmunological histamine and tryptase release from human cutaneous mast cells
Autore:
Veien, M; Szlam, F; Holden, JT; Yamaguchi, K; Denson, DD; Levy, JH;
Indirizzi:
Emory Univ Hosp, Dept Anesthesiol, Sch Med, Div Cardiothorac Anesthesiol &Crit Care, Atlanta, GA 30322 USA Emory Univ Hosp Atlanta GA USA 30322 ol &Crit Care, Atlanta, GA 30322 USA
Titolo Testata:
ANESTHESIOLOGY
fascicolo: 4, volume: 92, anno: 2000,
pagine: 1074 - 1081
SICI:
0003-3022(200004)92:4<1074:MONHAT>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-SKIN; ANAPHYLACTOID REACTIONS; MUSCLE-RELAXANTS; MEDIATOR RELEASE; SUBSTANCE-P; ANTI-IGE; DEGRANULATION; HETEROGENEITY; PURIFICATION; HYPOTENSION;
Keywords:
anaphylaxis; mediator release; phospholipases;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Levy, JH Emory Univ Hosp, Dept Anesthesiol, Sch Med, Div Cardiothorac Anesthesiol &Crit Care, 1364 Clifton Rd NE, Atlanta, GA 30322 USA Emory Univ Hosp 1364 Clifton Rd NE Atlanta GA USA 30322 30322 USA
Citazione:
M. Veien et al., "Mechanisms of nonimmunological histamine and tryptase release from human cutaneous mast cells", ANESTHESIOL, 92(4), 2000, pp. 1074-1081

Abstract

Background: If mast cells are stimulated they release multiple mediators that delineate markers for immunologic and nonimmunologic reactions; histamine and tryptase are the two best known. Although histamine can be assayed in plasma, it is a nonspecific marker with a very short half-life. Tryptase has a longer half-life, but its release has not been proven to be specific for anaphylaxis, The authors investigated the mechanisms of nonimmunologic histamine release from human cutaneous mast cells to understand the mechanisms of mediator release and to determine whether tryptase was specific for allergic mediated activation. Methods: Dispersed mast cell suspensions isolated from neonatal foreskins underwent challenge with vancomycin, calcium ionophore A23187, morphine, and atracurium, and histaminetryptase release was measured. The effects of calcium and magnesium, along with phospholipase C and phospholipase A(2) inhibitors, also were investigated. Results: Tryptase and histamine both were released by the known nonimmunologic stimuli (pharmacologic agents used in the current study; r(2) = 0.6), Furthermore, vancomycin- and atracurium-induced histamine release was calcium dependent. Phospholipase C and phospholipase A(2) inhibitors decreased vancomycin-induced histamine release bur not calcium ionophore A23187-induced release,Conclusions: Tryptase is not a specific marker of mast cell activation (i.e., anaphylaxis), and signaling mechanisms for mast cell activation Involveactivation of phospholipase C and phospholipase A(2) pathways that are also involved in other cellular activation mechanisms.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 02:15:42