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Titolo:
Ethanol improves decreased filterability of human red blood cells through modulation of intracellular signaling pathways
Autore:
Oonishi, T; Sakashita, K;
Indirizzi:
Kanto Teishin Hosp, Med Res Inst, NTT, Tokyo, Japan Kanto Teishin Hosp Tokyo Japan in Hosp, Med Res Inst, NTT, Tokyo, Japan
Titolo Testata:
ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH
fascicolo: 3, volume: 24, anno: 2000,
pagine: 352 - 356
SICI:
0145-6008(200003)24:3<352:EIDFOH>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; HUMAN-ERYTHROCYTES; MEMBRANE; SHAPE; DEFORMABILITY; SKELETAL; EXPOSURE;
Keywords:
ethanol; human red blood cell deformability; protein kinase A; protein kinase C; cytoskeletal system;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Oonishi, T Sankyo Co Ltd, Inst Res, Shinagawa Ku, 1-2-58 Hiromachi, Tokyo 1408710, Japan Sankyo Co Ltd 1-2-58 Hiromachi Tokyo Japan 1408710 8710, Japan
Citazione:
T. Oonishi e K. Sakashita, "Ethanol improves decreased filterability of human red blood cells through modulation of intracellular signaling pathways", ALC CLIN EX, 24(3), 2000, pp. 352-356

Abstract

Background: Deformability of red blood cells (RBCs) is one of the most important factors in maintaining the microcirculation system. In vitro studieshave revealed that ethanol seriously impairs the deformability of RBCs at concentrations far higher than those easily achievable in the blood after alcohol consumption. We examined the effect of ethanol on deformability at the physiologically achievable concentrations. Methods: To impair deformability quantitatively, washed RBCs from healthy young male volunteers were mechanically stressed by being injected rapidly through a fine needle. The deformability of RBCs was evaluated by measuringthe filterability through a nickel mesh filter. The activities of protein kinase A and protein kinase C in RBCs were measured by the ELISA method using an antibody for the phosphorylated substrate of the kinases. Results: Ethanol exhibited a biphasic effect on the mechanically impaired filterability of human RBCs, that is, an improvement at concentrations from0.02% to 0.2% (v/v) and a marked deterioration at 0.5% and higher. Withoutmechanical stress, ethanol decreased filterability at concentrations of 0.5% and higher. Ethanol increased the protein kinase A and protein kinase C activities of the mechanically stressed RBCs at concentrations of 0.02% andhigher and those of the RBCs without mechanical stress at concentrations of 0.05% and higher. Conclusions: The present results suggest a beneficial effect of ethanol onblood rheology at concentrations that are physiologically achievable in the blood and indicate the importance of intracellular signaling pathways in regulating the deformability of RBCs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 21:22:39