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Titolo:
Association of histone deacetylase with COUP-TF in tumorigenic Ad12-transformed cells and its potential role in shut-off of MHC class I transcription
Autore:
Smirnov, DA; Hou, SH; Ricciardi, RP;
Indirizzi:
Univ Penn, Sch Dent Med, Dept Microbiol, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 Microbiol, Philadelphia, PA 19104 USA Univ Penn, Sch Med, Dept Biochem & Biophys, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 & Biophys, Philadelphia, PA 19104 USA Univ Penn, Sch Arts & Sci, Dept Biol, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 Dept Biol, Philadelphia, PA 19104 USA
Titolo Testata:
VIROLOGY
fascicolo: 2, volume: 268, anno: 2000,
pagine: 319 - 328
SICI:
0042-6822(20000315)268:2<319:AOHDWC>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
HORMONE-RECEPTOR SUPERFAMILY; N-COR; ACTIVE REPRESSION; THYROID-HORMONE; EXPRESSION; GENE; COMPLEX; ENHANCER; BINDING; LEVEL;
Keywords:
COUP-TF; histone deacetylase; MHC class I; adenovirus-12;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Ricciardi, RP Univ Penn, Sch Dent Med, Dept Microbiol, Levy Res Bldg,Room 221,4010 Locust St, Philadelphia, PA 19104 USA Univ Penn Levy Res Bldg,Room221,4010 Locust St Philadelphia PA USA 19104
Citazione:
D.A. Smirnov et al., "Association of histone deacetylase with COUP-TF in tumorigenic Ad12-transformed cells and its potential role in shut-off of MHC class I transcription", VIROLOGY, 268(2), 2000, pp. 319-328

Abstract

Chicken ovalbumin upstream promoter-transcription factor (COUP-TF) is an orphan nuclear receptor that represses transcription of many genes. In adenovirus type 12 (Ad12) transformed cells, a high level of binding activity ofCOUP-TF to the major histocompatibility complex (MHC) class I enhancer correlates with the down-regulation of class I transcription, which, in turn, contributes to tumorigenesis. The mechanism by which COUP-TF represses transcription has yet to be elucidated. Here we show that COUP-TF represses transcription through its association with histone deacetylase. This was demonstrated using reciprocal binding assays that determined that the interaction between COUP-TF and histone deacetylase requires the COUP-TF C-terminal repression domain. Moreover, a histone deacetylase enzymatic activity was found to be associated with COUP-TF in Ad12-transformed cells. Transfection experiments further revealed that exogenous histone deacetylase facilitates transcriptional repression by COUP-TF Also, supershift assays suggest that the transcriptional corepressor N-CoR, which is known to associate with histone deacetylases, is a part of the COUP-TF complex bound to the MHC class I enhancer R2 site. Finally, we provide evidence that inhibition of histonedeacetylases relieves the repression of MHC class I expression in Ad12-transformed cells. Taken together these results support the notion that deacetylation of histones, mediated through COUP-TF, serves to down-regulate MHC class I transcription in AdlP-transformed cells. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 21:24:06