Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
C-fos mRNA induction in the central and peripheral nervous systems of diisopropyl phosphorofluoridate (DFP)-treated hens
Autore:
Gupta, RP; Damodaran, TV; Abou-Donia, MB;
Indirizzi:
Duke Univ, Med Ctr, Dept Pharmacol & Canc Biol, Durham, NC 27708 USA Duke Univ Durham NC USA 27708 Pharmacol & Canc Biol, Durham, NC 27708 USA
Titolo Testata:
NEUROCHEMICAL RESEARCH
fascicolo: 3, volume: 25, anno: 2000,
pagine: 327 - 334
SICI:
0364-3190(200003)25:3<327:CMIITC>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
INDUCED DELAYED NEUROTOXICITY; SCIATIC-NERVE; CYTOSKELETAL PROTEINS; TRANSCRIPTION FACTOR; GENE-EXPRESSION; RAT-BRAIN; TRANSECTION; ACTIVATION; RECEPTORS;
Keywords:
diisopropyl phosphorofluoridate; c-fos; brain; spinal cord; sciatic nerve; chicken;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Abou-Donia, MB Duke Univ, Med Ctr, Dept Pharmacol & Canc Biol, POB 3813, Durham, NC 27708USA Duke Univ POB 3813 Durham NC USA 27708 Durham, NC 27708USA
Citazione:
R.P. Gupta et al., "C-fos mRNA induction in the central and peripheral nervous systems of diisopropyl phosphorofluoridate (DFP)-treated hens", NEUROCHEM R, 25(3), 2000, pp. 327-334

Abstract

A single dose of diisopropyl phosphorofluoridate (DFP), an organophosphorus ester, produces delayed neurotoxicity (OPIDN) in hen. DFP produces mild ataxia in hens in 7-14 days, which develops into severe ataxia or paralysis as the disease progresses. Since, OPIDN is associated with alteration in the expression of several proteins (e.g., Ca2+/calmodulin-dependent protein kinase II (CaM kinase II) alpha-subonit, tau, tubulin, neurofilament (NF) protein, vimentin, GFAP) as well as their mRNAs (e.g., NF, CaM kinase II alpha-subunit), we determined the effect of a single dose of DFP on the expression of one of the best known immediate-early gene (IEG), c-fos. C-fos expression was measured by Northern hybridization in cerebrum, cerebellum, brainstem, midbrain, spinal cord, and the sciatic nerves of hens at 0.5 hr, 1 hr, 2 hr, 1 day, 5 days, 10 days, and 20 days after a single 1.7 mg/kg, sc. injection of DFP. All the tissues (cerebrum, 52%; cerebellum, 55%; brainstem, 49%; midbrain, 23%; spinal cord, 80%; sciatic nerve, 157%) showed significant increase in c-fos expression in 30 min and this elevated level persisted at least up to 2 hr. Expressions of beta-actin mRNA and 18S RNA were used as internal controls. The significant increase in c-fos expression in DFP-treated hens suggests that c-fos may be one of the IEGs involved in the development of OPIDN.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 13:11:18