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Titolo:
Differential superactivation of adenylyl cyclase isozymes after chronic activation of the CB1 cannabinoid receptor
Autore:
Rhee, MH; Nevo, I; Avidor-Reiss, T; Levy, R; Vogel, Z;
Indirizzi:
Weizmann Inst Sci, Dept Neurobiol, IL-76100 Rehovot, Israel Weizmann Inst Sci Rehovot Israel IL-76100 biol, IL-76100 Rehovot, Israel
Titolo Testata:
MOLECULAR PHARMACOLOGY
fascicolo: 4, volume: 57, anno: 2000,
pagine: 746 - 752
SICI:
0026-895X(200004)57:4<746:DSOACI>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
BETA-GAMMA-SUBUNITS; CHRONIC OPIOID TREATMENT; RAT-BRAIN; INHIBITORY RECEPTORS; INVERSE AGONIST; DOWN-REGULATION; BINDING; CELLS; DELTA-9-TETRAHYDROCANNABINOL; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Vogel, Z Weizmann Inst Sci, Dept Neurobiol, IL-76100 Rehovot, Israel Weizmann Inst Sci Rehovot Israel IL-76100 76100 Rehovot, Israel
Citazione:
M.H. Rhee et al., "Differential superactivation of adenylyl cyclase isozymes after chronic activation of the CB1 cannabinoid receptor", MOLEC PHARM, 57(4), 2000, pp. 746-752

Abstract

Many types of cells exhibit increased adenylyl cyclase (AC) activity afterchronic agonist treatment of G(i/o)-coupled receptors. This phenomenon, defined as AC superactivation or sensitization, has mostly been studied for the opioid receptors and is implicated in opiate addiction. Here we show that this phenomenon is also observed on chronic activation of the CB1 cannabinoid receptor. Moreover, using COS-7 cells cotransfected with CB1 receptor and individual AC isozymes, we could show selective superactivation of AC types I, III, V, VI, and VIII. The level of superactivation was dependent onthe concentration of agonist and time of agonist exposure and was not dependent on the AC stimulator used. No superactivation of AC types II, IV, or VII was observed in COS-7 cells cotransfected with CB1. The superactivationof AC type V was abolished by pretreatment with pertussis toxin and by cotransfection with the carboxy terminus of beta-adrenergic receptor kinase, which serves as a scavenger of G(beta gamma) dimers, implying a role for theG(i/o) proteins and especially G(beta gamma) dimers in the cannabinoid-induced superactivation of AC.

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Documento generato il 10/07/20 alle ore 02:50:36