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Titolo:
Isolation of shiga toxin-producing Escherichia coli (STEC) from foods using EHEC agar
Autore:
Hudson, JA; Nicol, C; Capill, J; Bennett, J;
Indirizzi:
ESR Ltd, Christchurch Sci Ctr, Christchurch, New Zealand ESR Ltd Christchurch New Zealand rch Sci Ctr, Christchurch, New Zealand ESR Ltd, Kenepuru Sci Ctr, Porirua, New Zealand ESR Ltd Porirua New Zealand Ltd, Kenepuru Sci Ctr, Porirua, New Zealand
Titolo Testata:
LETTERS IN APPLIED MICROBIOLOGY
fascicolo: 2, volume: 30, anno: 2000,
pagine: 109 - 113
SICI:
0266-8254(200002)30:2<109:IOSTEC>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMOLYTIC-UREMIC SYNDROME; ENTEROHEMOLYSIN; IDENTIFICATION; STRAINS; EAEA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Hudson, JA ESR Ltd, Christchurch Sci Ctr, POB 29-181, Christchurch, New Zealand ESR Ltd POB 29-181 Christchurch New Zealand urch, New Zealand
Citazione:
J.A. Hudson et al., "Isolation of shiga toxin-producing Escherichia coli (STEC) from foods using EHEC agar", LETT APPL M, 30(2), 2000, pp. 109-113

Abstract

Enterohaemorrhagic Escherichia coli (EHEC) agar was evaluated for its ability to recover one isolate of each of three serotypes (O157:H7, O26 and O113:H21) of shiga toxin-producing E. coli (STEC) from raw mince, pasteurized milk and salami after enrichment. The method detected around one colony-forming unit (cfu) in 25 ml in milk, but was less sensitive with salami, requiring 10-1000 cfu 25 g(-1) (depending on serotype) for detection. In raw minced beef any enterohaemolysin-producing colonies were outnumbered by other colonies and only one of 12 enrichments yielded the inoculum serotype. Additional tests were conducted on 15 retail meat products. One 25-g sample of each product was processed as purchased, while another was inoculated with 157-185 cfu of a cocktail of E. coli O157, O113 and O26 cultures. Recovery was easily achieved with cooked meat products and salami. Recovery from rawminced meat was again difficult, but sometimes possible. Testing more suspect colonies than were tested in this study would presumably increase the sensitivity of the method.

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Documento generato il 17/01/21 alle ore 17:24:10