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Titolo:
Upregulation of myogenin by N-cadherin adhesion in three-dimensional cultures of skeletal myogenic BHK cells
Autore:
Seghatoleslami, MR; Myers, L; Knudsen, KA;
Indirizzi:
Lankenau Med Res Ctr, Wynnewood, PA 19096 USA Lankenau Med Res Ctr Wynnewood PA USA 19096 Ctr, Wynnewood, PA 19096 USA
Titolo Testata:
JOURNAL OF CELLULAR BIOCHEMISTRY
fascicolo: 2, volume: 77, anno: 2000,
pagine: 252 - 264
SICI:
0730-2312(200003)77:2<252:UOMBNA>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
MUSCLE DIFFERENTIATION; SIGNAL-TRANSDUCTION; MYOD FAMILY; TRANSCRIPTION FACTORS; UPSTREAM SEQUENCES; MOUSE EMBRYOS; BETA-CATENIN; R-CADHERIN; LIMB BUD; EXPRESSION;
Keywords:
cadherins; cell-cell adhesion; myogenin; skeletal myogenesis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
69
Recensione:
Indirizzi per estratti:
Indirizzo: Knudsen, KA Lankenau Med Res Ctr, 100 Lancaster Ave, Wynnewood, PA 19096 USA Lankenau Med Res Ctr 100 Lancaster Ave Wynnewood PA USA 19096
Citazione:
M.R. Seghatoleslami et al., "Upregulation of myogenin by N-cadherin adhesion in three-dimensional cultures of skeletal myogenic BHK cells", J CELL BIOC, 77(2), 2000, pp. 252-264

Abstract

Cells of the baby hamster kidney (BHK) line express the skeletal muscle determining transcription factor MyoD but fail to differentiate. Unlike most skeletal myogenic cells, which express multiple members of the cadherin family of cell-cell adhesion proteins, the BHK cells lack a robust cadherin adhesion system. We previously published that forced expression of N- (or E)-cadherin in BHK cells increases the level of endogenous catenins, mediates strong cell-cell adhesion, and enhances differentiation of BHK cells induced to differentiate by placing them in three-dimensional (3-D) culture (Redfield et al. [1997] J. Cell. Biol. 138:1323-1331). This report demonstrates that N-cadherin adhesion upregulates the protein level of nuclear myogenin in cells induced to differentiate by 3-D culture. Myogenin is a transcription factor required for differentiation of skeletal muscle. It was not detected in monolayer culture, whether the cells expressed N-cadherin or not, nor was it upregulated in 3-D cultures of cells lacking N-cadherin. The activity of two myogenin-chloramphenicol acetyltransferase (CAT) reporter constructs containing 3.7 or 1.1 kb upstream regulatory region of the mouse myogenin gene was increased significantly in N-cadherin-expressing cells inducedto differentiate by 3-D culture. Our observations indicate that N-cadherinadhesion stimulates skeletal myogenesis by upregulating myogenin. (C) 2000Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 23:18:45