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Titolo:
Protective effect of benidipine against the abnormal electrical activity in single ventricular myocytes of the guinea pig under simulated ischemic conditions and reperfusion
Autore:
Tokuno, T; Muraki, K; Watanabe, M; Imaizumi, Y;
Indirizzi:
Nagoya City Univ, Fac Pharmaceut Sci, Dept Pharmacol & Therapeut, Mizuho Ku, Nagoya, Aichi 4678603, Japan Nagoya City Univ Nagoya Aichi Japan 4678603 Nagoya, Aichi 4678603, Japan Nagoya City Univ, Fac Pharmaceut Sci, Dept Chem Pharmacol, Mizuho Ku, Nagoya, Aichi 4678603, Japan Nagoya City Univ Nagoya Aichi Japan 4678603 Nagoya, Aichi 4678603, Japan
Titolo Testata:
JAPANESE JOURNAL OF PHARMACOLOGY
fascicolo: 3, volume: 82, anno: 2000,
pagine: 199 - 209
SICI:
0021-5198(200003)82:3<199:PEOBAT>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
SENSITIVE K+ CHANNELS; MYOCARDIAL-ISCHEMIA; INTRACELLULAR CALCIUM; CONTRACTILE FAILURE; CARDIAC MYOCYTES; MECHANISMS; HEART; CELLS; ARRHYTHMIAS; ANOXIA;
Keywords:
heart; ischemia; reperfusion; electrical abnormality; benidipine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Imaizumi, Y Nagoya City Univ, Fac Pharmaceut Sci, Dept Pharmacol & Therapeut, Mizuho Ku, 3-1 Tanabedori, Nagoya, Aichi 4678603, Japan Nagoya City Univ 3-1 Tanabedori Nagoya Aichi Japan 4678603 pan
Citazione:
T. Tokuno et al., "Protective effect of benidipine against the abnormal electrical activity in single ventricular myocytes of the guinea pig under simulated ischemic conditions and reperfusion", JPN J PHARM, 82(3), 2000, pp. 199-209

Abstract

Induction of electrical abnormalities (EAs) under simulated ischemic conditions and after reperfusion was measured from single cardiac myocytes isolated from guinea pig ventricle using whole-cell voltage or current clamp with perforated patch variation. Conditions of simulated ischemia were produced by the exchange of medium from the standard one oxygenated with 95% O-2-5% CO2 gas (pH 7.4) to the modified one, which contained no glucose, 8 mM Kand 30 mM sodium-D,L-lactate and was gassed with 90% argon - 10% CO2 (pH 6.6). Under the simulated ischemia for 20 min, EAs such as delayed afterdepolarization, early afterdepolarization, automatic activity or transient inward current were observed in about 37% of myocytes driven electrically at 1 Hz. Irreversible hypercontracture occurred in myocytes of 10% or less. Uponreperfusion with the standard solution, EAs and hypercontracture were observed in about 43% and 22% of cells, respectively. Glibenclamide-sensitive current was detected during ischemia, but tended to be enhanced during reperfusion. Amplitude of Ca2+ current and ATP-sensitive K+ current after reperfusion varied widely with time and from cell to cell. When myocytes were pretreated for 10 min with 10 nM benidipine, a 1,4-dihydropyridine derivative Ca2+ blocker, the incidence of EAs and hypercontracture was markedly reduced, suggesting the protective effect of benidipine against cardiac cell injury during ischemia and reperfusion.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 16:35:12