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Titolo:
Partial cloning and sequencing of chick fibrillin-1 cDNA
Autore:
Zhou, GM; Price, CE; Rosenquist, TH; Gadson, PF; Godfrey, M;
Indirizzi:
Univ Nebraska, Med Ctr, Munroe Ctr Human Genet, Omaha, NE 68198 USA Univ Nebraska Omaha NE USA 68198 roe Ctr Human Genet, Omaha, NE 68198 USA Univ Nebraska, Med Ctr, Dept Cell Biol & Anat, Omaha, NE 68198 USA Univ Nebraska Omaha NE USA 68198 pt Cell Biol & Anat, Omaha, NE 68198 USA Univ Nebraska, Med Ctr, Dept Pediat, Omaha, NE 68198 USA Univ Nebraska Omaha NE USA 68198 ed Ctr, Dept Pediat, Omaha, NE 68198 USA
Titolo Testata:
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
fascicolo: 1, volume: 36, anno: 2000,
pagine: 19 - 25
SICI:
1071-2690(200001)36:1<19:PCASOC>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
ELASTIN-ASSOCIATED MICROFIBRILS; DEVELOPING CARDIOVASCULAR-SYSTEM; CARDIAC NEURAL CREST; AORTIC-ARCH ARTERY; AVIAN EMBRYO; EXTRACELLULAR-MATRIX; MARFAN-SYNDROME; SMOOTH-MUSCLE; HUMAN GENE; GROWTH;
Keywords:
fibrillin; chick; development; in situ hybridization; extracellular matrix;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Godfrey, M Univ Nebraska, Med Ctr, Nebraska Med Ctr 985430, Omaha, NE 68198 USA Univ Nebraska Omaha NE USA 68198 r 985430, Omaha, NE 68198 USA
Citazione:
G.M. Zhou et al., "Partial cloning and sequencing of chick fibrillin-1 cDNA", IN VITRO-AN, 36(1), 2000, pp. 19-25

Abstract

The recent identification of numerous matrix genes and gene products has allowed a detailed examination of their roles in development. Two of these extracellular matrix proteins, fibrillin-1 and fibrillin-2, are components of the elastin-associated microfibrils. Given what is known about the distribution of the fibrillins in normal tissues and the abnormalities that result when mutations occur, a basic hypothesis has emerged: fibrillin-1 is primarily responsible for load bearing and providing structural integrity, whereas fibrillin-2 may be a director of elastogenesis. Nevertheless, examination of phenotypes in disorders caused by mutations in fibrillin-1 or fibrillin-2 suggests some common functions. To better understand these similar anddiverse roles, it would be helpful to examine these proteins during chick development. To accomplish this goal, it is first necessary to characterizethe chick homologs of the known fibrillins. In this study, the partial chick FBN1 cDNA was identified by polymerase chain reaction-aided cloning as afirst step toward elucidating these goals. Sequence analysis indicated that there is striking conservation between chick and mammalian fibrillin-1 atthe DNA and protein levels. Antisense and sense riboprobes were synthesized and used in in situ hybridization in stage 14 chick embryos and high levels of FBN1 transcripts were observed in the heart.

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Documento generato il 23/09/20 alle ore 12:26:08