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Titolo:
Detection of apoptosis in a heterogenous cell population using flow cytometry
Autore:
Sedlak, J; Hunakova, L; Duraj, J; Sulikova, M; Chovancova, J; Novotny, L; Chorvath, B;
Indirizzi:
Slovak Acad Sci, Canc Res Inst, Dept Mol Immunol, Bratislava 83391, Slovakia Slovak Acad Sci Bratislava Slovakia 83391 ol, Bratislava 83391, Slovakia
Titolo Testata:
GENERAL PHYSIOLOGY AND BIOPHYSICS
, volume: 18, anno: 1999,
pagine: 147 - 154
SICI:
0231-5882(199910)18:<147:DOAIAH>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
LABELED ANNEXIN-V; EXPRESSION; LEUKEMIA; PROTEIN; CYCLE; PHOSPHATIDYLSERINE; QUANTIFICATION; INHIBITOR; INDUCTION; DEATH;
Keywords:
apoptosis; flow cytometry; "hypodiploid" DNA content; annexin V binding; Apo 2.7 monoclonal antibody; scatter parameters; DNA "ladder";
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Sedlak, J Slovak Acad Sci, Canc Res Inst, Dept Mol Immunol, Vlarska 7, Bratislava 83391, Slovakia Slovak Acad Sci Vlarska 7 Bratislava Slovakia 83391 1, Slovakia
Citazione:
J. Sedlak et al., "Detection of apoptosis in a heterogenous cell population using flow cytometry", GEN PHYSL B, 18, 1999, pp. 147-154

Abstract

Apoptosis induced in human leukemic cells (promyelocytic human leukemic cells HL-60, multidrug-resistant subline HL-60/VCR) and human ovarian carcinoma cells (A2780 and multidrug-resistant subline A2780/ADR) in vitro was detected by flow cytometric analysis or DNA electrophoresis. The cytofluorometric techniques utilized, i. e. detection of phosphatidylserine exposed at the outer surface of the plasma membrane, identification of cells with "sub-G(O)" DNA content or increased light side scatter (cell internal structure)correlated with the electrophoretic determination of DNA fragmentation ("DNA ladder"). Detection of the 34 kDa mitochondrial protein recognized by the monoclonal antibody Apo2.7 yielded elevated percentages of apoptotic cells, suggesting that this technique detecting both early and late apoptosis in digitonin-fixed cells might not be restricted to the specific detection of programmed cell death.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 19:19:20