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Titolo:
The rate at which human sperm are immobilized and killed by mild acidity
Autore:
Olmsted, SS; Dubin, NH; Cone, RA; Moench, TR;
Indirizzi:
Johns Hopkins Univ, Dept Biophys, Baltimore, MD 21218 USA Johns Hopkins Univ Baltimore MD USA 21218 iophys, Baltimore, MD 21218 USA Union Mem Hosp, Dept Obstet & Gynecol, Baltimore, MD USA Union Mem Hosp Baltimore MD USA Dept Obstet & Gynecol, Baltimore, MD USA ReProtect LLC, Baltimore, MD USA ReProtect LLC Baltimore MD USAReProtect LLC, Baltimore, MD USA
Titolo Testata:
FERTILITY AND STERILITY
fascicolo: 4, volume: 73, anno: 2000,
pagine: 687 - 693
SICI:
0015-0282(200004)73:4<687:TRAWHS>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTRACELLULAR PH; CYTOPLASMIC PH; MOTILITY; NONOXYNOL-9;
Keywords:
sperm; motility; pH; BufferGel; contraceptive; intracellular pH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Cone, RA Johns Hopkins Univ, Dept Biophys, 3400 N Charles St, Baltimore, MD 21218 USA Johns Hopkins Univ 3400 N Charles St Baltimore MD USA 21218 8 USA
Citazione:
S.S. Olmsted et al., "The rate at which human sperm are immobilized and killed by mild acidity", FERT STERIL, 73(4), 2000, pp. 687-693

Abstract

Objective: To determine the rate at which mild acidity immobilizes and kills human sperm and to evaluate an acidic microbicide, BufferGel, for sperm immobilization. Design: Controlled in vitro study. Setting: An academic research university and hospital andrology lab. Patient(s): Eight volunteer male sperm donors. Intervention(s): Semen samples were treated with hydrochloric acid (HCl) or BufferGel. Main Outcome Measure(s): Sperm motility was measured by using a computerized automated semen analyzer and video microscopy. Sperm membrane permeability and intracellular pH were measured by using fluorescent techniques. Result(s): In semen acidified with HCl to ptr 4.0, sperm were rapidly immobilized (within 1 min) and were irreversibly immobilized (killed) within 10minutes. The speed of immobilization and of killing were both linearly proportional to hydrogen ion activity over a pH range of 7.5-4.0. Across the same range, the intracellular pH of human sperm equilibrated to within 0.5 pH units of extracellular pH within 1-2 minutes. BufferGel immobilized spermsignificantly faster than HCl from pH 4.0-6.0. Conclusion(s): Exposure to mild acidity rapidly acidifies the intracellular pH of human sperm and is rapidly spermicidal. BufferGel accelerates acid immobilization of sperm.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 12:12:22