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Titolo:
Contribution of proliferating leukocytes to phenotypic change in smooth muscle cells during the development of coronary arteriosclerosis in transplanted hearts
Autore:
Shirasawa, B; Hamano, K; Ueda, M; Ito, H; Kobayashi, T; Fujimura, Y; Kojima, A; Esato, K;
Indirizzi:
Yamaguchi Univ, Sch Med, Dept Surg 1, Ube, Yamaguchi 7558505, Japan Yamaguchi Univ Ube Yamaguchi Japan 7558505 Ube, Yamaguchi 7558505, Japan Osaka City Univ, Sch Med, Dept Pathol 2, Osaka 558, Japan Osaka City UnivOsaka Japan 558 Sch Med, Dept Pathol 2, Osaka 558, Japan Osaka City Univ Sch, Fac Human Life Sci, Dept Food & Nutr, Osaka, Japan Osaka City Univ Sch Osaka Japan ife Sci, Dept Food & Nutr, Osaka, Japan
Titolo Testata:
EUROPEAN SURGICAL RESEARCH
fascicolo: 1, volume: 32, anno: 2000,
pagine: 30 - 38
SICI:
0014-312X(200001/02)32:1<30:COPLTP>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN ATHEROSCLEROTIC PLAQUES; HUMAN RENAL-ALLOGRAFTS; NUCLEAR ANTIGEN PCNA; MONOCLONAL-ANTIBODY; IMMUNOCYTOCHEMICAL ANALYSIS; CHRONIC REJECTION; CELLULAR COMPOSITION; HUMAN-TISSUES; MACROPHAGE; EXPRESSION;
Keywords:
heart transplantation; coronary artery; arteriosclerosis; transplant rejection; smooth muscle cell;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Hamano, K Yamaguchi Univ, Sch Med, Dept Surg 1, Ube, Yamaguchi 7558505, Japan Yamaguchi Univ Ube Yamaguchi Japan 7558505 guchi 7558505, Japan
Citazione:
B. Shirasawa et al., "Contribution of proliferating leukocytes to phenotypic change in smooth muscle cells during the development of coronary arteriosclerosis in transplanted hearts", EUR SURG RE, 32(1), 2000, pp. 30-38

Abstract

Background: It is well known that coronary arteriosclerosis after heart transplantation is concentric and rich in smooth muscle cells (SMCs); however, the role played by rejection in the intimal thickening caused by SMCs in coronary arteriosclerosis remains unclear. In this study, we examined the process of intimal hyperplasia caused by SMCs and evaluated the relationshipbetween the differentiation state of SMCs and local inflammation caused byrejection. Methods: Lewis rat hearts were heterotopically transplanted into F344 rats (allotransplantation group) or other Lewis rats (isotransplantation group). Cyclosporin A (5 mg/kg/day) was injected intramuscularly for 20 days after transplantation in both groups. The transplanted hearts were examined immunohistochemically using several monoclonal antibodies; namely, HHF-35, CGA7, vimentin, a-actin, HIS36, R73 and proliferating cell nuclear antigen (PCNA). To evaluate the degree of local immunological response caused by rejection, the anti-PCNA antibody was used. To reveal the subtypes ofproliferating cells in the thickened intima, HIS36 and R73 antibodies wereused. Results: In the allotransplantation group, SMCs in the media began to undergo a phenotypic change toward a poorly differentiated state 30 days after transplantation. Intimal hyperplasia was observed 60 days after transplantation, the thickened intima being composed mainly of dedifferentiated SMCs with abundant PCNA(+), most of which were macrophages and T cells. Thestate of differentiation of SMCs in the thickened intima 90 days after transplantation varied from a dedifferentiated to a highly differentiated state. These changes were strongly correlated with the expression of PCNA. Conclusion: The expression of PCNA was strongly correlated with the differentiation state of SMCs. Thus, local inflammation caused by rejection may play an important role in the initiation of phenotypic change in SMCs. Copyright (C) 2000 S. Karger AG. Basel.

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Documento generato il 25/11/20 alle ore 01:10:29