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Titolo:
Putative fusogenic activity of NSF is restricted to a lipid mixture whose coalescence is also triggered by other factors
Autore:
Brugger, B; Nickel, W; Weber, T; Parlati, F; McNew, JA; Rothman, JE; Sollner, T;
Indirizzi:
Mem Sloan Kettering Canc Ctr, Cellular Biochem & Biophys Program, New York, NY 10021 USA Mem Sloan Kettering Canc Ctr New York NY USA 10021 New York, NY 10021 USA
Titolo Testata:
EMBO JOURNAL
fascicolo: 6, volume: 19, anno: 2000,
pagine: 1272 - 1278
SICI:
0261-4189(20000315)19:6<1272:PFAONI>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
SENSITIVE FUSION PROTEIN; PHOSPHATIDYLSERINE-CONTAINING VESICLES; MEMBRANE-FUSION; IN-VITRO; ENDOPLASMIC-RETICULUM; VESICULAR TRANSPORT; ELECTRON-MICROSCOPY; SNARE COMPLEX; ALPHA-SNAP; T-SNARE;
Keywords:
fusion; lipid; NSF; SNARE; vesicle;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Sollner, T Mem Sloan Kettering Canc Ctr, Cellular Biochem & Biophys Program, 1275 York Ave, New York, NY 10021 USA Mem Sloan Kettering Canc Ctr 1275 York Ave New York NY USA 10021
Citazione:
B. Brugger et al., "Putative fusogenic activity of NSF is restricted to a lipid mixture whose coalescence is also triggered by other factors", EMBO J, 19(6), 2000, pp. 1272-1278

Abstract

It has recently been reported that N-ethylmaleimidesensitive fusion ATPase(NSF) can fuse protein-free liposomes containing substantial amounts of 1,2-dioleoylphosphatidylserine (DOPS) and 1,2-dioleoyl-phosphatidyl-ethanolamine (DOPE) (Otter-Nilsson et al., 1999), The authors impart physiological significance to this observation and propose to re-conceptualize the generalrole of NSF in fusion processes. We can confirm that isolated NSF can fuseliposomes of the specified composition. However, this activity of NSF is resistant to inactivation by N-ethylmaleimide and does not depend on the presence of alpha-SNAP (soluble NSF-attachment protein). Moreover, under the same conditions, either alpha-SNAP, other proteins apparently unrelated to vesicular transport (glyceraldehyde-3-phosphate dehydrogenase or lactic dehydrogenase) or even 3 mM magnesium ions can also cause lipid mixing. In contrast, neither NSF nor the other proteins nor magnesium had any significant fusogenic activity with liposomes composed of a biologically occurring mixture of lipids. A straightforward explanation is that the lipid composition chosen as optimal for NSF favors non-specific fusion because it is physically unstable when formed into liposomes, A variety of minor perturbations could then trigger coalescence.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 03:16:52