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Titolo:
Cloning, sequencing and structural analysis of a pea cDNA encoding EF-1 alpha
Autore:
Cong, XY; Ling, SY; Zhu, YX;
Indirizzi:
Peking Univ, Coll Life Sci, Natl Lab Prot Engn & Plant Genet Engn, Beijing100871, Peoples R China Peking Univ Beijing Peoples R China 100871 eijing100871, Peoples R China
Titolo Testata:
CHINESE SCIENCE BULLETIN
fascicolo: 4, volume: 45, anno: 2000,
pagine: 338 - 343
SICI:
1001-6538(200002)45:4<338:CSASAO>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
ELONGATION-FACTOR 1-ALPHA; MAIZE ENDOSPERM; FACTOR-1-ALPHA; PROTEIN; EXPRESSION; CALMODULIN;
Keywords:
pea cDNA Library; EF-1 alpha; sequence analysis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Zhu, YX Peking Univ, Coll Life Sci, Natl Lab Prot Engn & Plant Genet Engn,Beijing100871, Peoples R China Peking Univ Beijing Peoples R China 100871 0871, Peoples R China
Citazione:
X.Y. Cong et al., "Cloning, sequencing and structural analysis of a pea cDNA encoding EF-1 alpha", CHIN SCI B, 45(4), 2000, pp. 338-343

Abstract

A cDNA library with the capacity of 2.0 X 10(6) was constructed using mRNAextracted, from gibberellic acid (GA)-treated G2 pea seedlings and a 1.6 kb cDNA with 100 nucleotides of 5' non-coding region and 223 nucleotides of 3' non-coding region was obtained by random screening. The DNA fragment contains an open reading frame of 1 344 nucleotides and encodes 447 amino acids. Sequence analysis of DNA and deduced polypeptide demonstrated that it encoded for a pea elongation factor 1-alpha (EF-1 alpha). The functional domains of EF-1 alpha is well conserved and EF-1 alpha can be a good candidate for studying molecular evolution.

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Documento generato il 10/07/20 alle ore 09:48:21