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Titolo:
Spectinomycin inhibits the self-splicing of the group 1 intron RNA
Autore:
Park, IK; Kim, JY; Lim, EH; Shin, S;
Indirizzi:
Koran Sahmyook Univ, Fac Sci, Dept Biol, Seoul 139742, South Korea Koran Sahmyook Univ Seoul South Korea 139742 , Seoul 139742, South Korea Dongguk Univ, Fac Sci, Dept Biol, Seoul 100715, South Korea Dongguk Univ Seoul South Korea 100715 pt Biol, Seoul 100715, South Korea
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 2, volume: 269, anno: 2000,
pagine: 574 - 579
SICI:
0006-291X(20000316)269:2<574:SITSOT>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROUP-I INTRON; THYMIDYLATE SYNTHASE GENE; ANTIBIOTIC INHIBITION; INTERVENING SEQUENCE; RIBOZYME;
Keywords:
T4 phage td intron; spectinomycin; RNA splicing; inhibition;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Park, IK Dongguk Univ, Dept Appl Biol, Seoul 100715, South Korea Dongguk Univ Seoul South Korea 100715 Seoul 100715, South Korea
Citazione:
I.K. Park et al., "Spectinomycin inhibits the self-splicing of the group 1 intron RNA", BIOC BIOP R, 269(2), 2000, pp. 574-579

Abstract

Effects of the aminoglycoside spectinomycin on the self-splicing of primary transcripts of the phage T4 thymidylate synthase gene (td) have been investigated. The kinetic analysis demonstrated that spectinomycin acts as a mixed noncompetitive inhibitor for the td intron RNA with a K-i of 7.2 mM. Increasing the spectinomycin concentration raised the K-m values with the corresponding decrease of V-max and k(cat) values. The specificity of the splicing inhibition by spectinomycin is due to changes in both K-m and k(cat). The splicing inhibition by spectinomycin is dependent on pH changes and Mg2 concentration, indicating electrostatic interactions with the intron RNA. It has been proposed that the key structural features in spectinomycin responsible for the inhibition of splicing may be the hydroxyl groups on the antibiotic. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/04/20 alle ore 02:21:36