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Titolo:
A high-level expression of CYP2A in the lung of the suncus (Suncus murinus) and its role in the activation of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone
Autore:
Mushiroda, T; Ariyoshi, N; Kimura, K; Takahara, E; Nagata, O; Kato, H; Kamataki, T;
Indirizzi:
Hokkaido Univ, Grad Sch Pharmaceut Sci, Div Pharmacobiodynam, Lab Drug Metab,Kita Ku, Sapporo, Hokkaido 0600812, Japan Hokkaido Univ Sapporo Hokkaido Japan 0600812 oro, Hokkaido 0600812, Japan Sumitomo Pharmaceut Co Ltd, Clin Res & Dev Div, Osaka 5548558, Japan Sumitomo Pharmaceut Co Ltd Osaka Japan 5548558 Div, Osaka 5548558, Japan Hokuriku Seiyaku Co, Div Res & Dev, Fukui 9118555, Japan Hokuriku Seiyaku Co Fukui Japan 9118555 Res & Dev, Fukui 9118555, Japan
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 2, volume: 269, anno: 2000,
pagine: 393 - 396
SICI:
0006-291X(20000316)269:2<393:AHEOCI>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN LIVER-MICROSOMES; COUMARIN 7-HYDROXYLATION; CYTOCHROMES P450; RAT LUNG; ENZYMES; CELLS; NNK;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Kamataki, T Hokkaido Univ, Grad Sch Pharmaceut Sci, Div Pharmacobiodynam, Lab Drug Metab,Kita Ku, N12W6, Sapporo, Hokkaido 0600812, Japan Hokkaido Univ N12W6 Sapporo Hokkaido Japan 0600812 0812, Japan
Citazione:
T. Mushiroda et al., "A high-level expression of CYP2A in the lung of the suncus (Suncus murinus) and its role in the activation of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone", BIOC BIOP R, 269(2), 2000, pp. 393-396

Abstract

Northern blot analysis of mRNA prepared from the lung of Suncus murinus (suncus), which was classified as an ancestor of primates, revealed that the expression level of cytochrome P450 2A (CYP2A) mRNA was about 100-fold higher than in the lung from rats and mice. To confirm that the pulmonary CYP2Aof the suncus had a catalytic activity, the metabolism of a specific substrate for CYP2A6, (+)-cis-3,5-dimethyl-2-(3-pyridyl) thiazolidin-4-one hydrochloride (SM-12502), was determined. The intrinsic clearance for SM-12502 S-oxidation by the suncus lung microsomes was calculated to be 99-fold higher than that by rat liver microsomes. The mutagen-producing activity of a 9000g supernatant fraction prepared from suncus lung was examined using 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) as a promutagen. The results showed that the suncus lung possessed 82-fold higher mutagen-producing activity than the rat lung, indicating that NNK was efficiently activated by the CYP2A isoform expressed in the suncus lung and that the suncus was a sensitive animal species to the genotoxicity of NNK contained in tobacco smoke. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 00:00:17