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Titolo:
Determination of a new asiatic acid derivative, AS 2-006A in rat plasma and urine, and human plsma by high-performance liquid chromatography
Autore:
Kim, GB; Kim, YG; Jew, SS; Lee, MG;
Indirizzi:
Seoul Natl Univ, Coll Pharm, Kwanak Gu, Seoul 151742, South Korea Seoul Natl Univ Seoul South Korea 151742 k Gu, Seoul 151742, South Korea
Titolo Testata:
RESEARCH COMMUNICATIONS IN MOLECULAR PATHOLOGY AND PHARMACOLOGY
fascicolo: 3, volume: 104, anno: 1999,
pagine: 339 - 344
SICI:
1078-0297(1999)104:3<339:DOANAA>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
5
Recensione:
Indirizzi per estratti:
Indirizzo: Lee, MG Seoul Natl Univ, Coll Pharm, Kwanak Gu, San 56-1,Shinlim Dong, Seoul 151742, South Korea Seoul Natl Univ San 56-1,Shinlim Dong Seoul South Korea 151742 ea
Citazione:
G.B. Kim et al., "Determination of a new asiatic acid derivative, AS 2-006A in rat plasma and urine, and human plsma by high-performance liquid chromatography", RES COM M P, 104(3), 1999, pp. 339-344

Abstract

A high-performance liquid chromatographic method was developed for the determination of a new wound healing agent, AS 2-006A (ethoxymethyl 2-oxo-3, 23-O-isopropylideneasiatate), in rat plasma and urine, and human plasma. Thesample preparation was simple: 2 volumes of acetonitrile were added to thebiological samples to deproteinize it. A 50-mu l aliquot of the supernatant was injected onto the reversed-phase column. The mobile phase employed was acctonitrile : H2O (9:1, v/v) and run at a flow rate of 1.1 ml/min. The column effluent was monitored by a UV detector set at 205 nm. The retention time for AS 2-006A was approximately 29.5 min. The detection limits for AS 2-006A in rat and human plasma were both 1 mu g/ml, and in rat urine was 2 mu g/ml. The coefficients of variation of the assay (within-day and between-day) were generally low (below 10.8%) for rat plasma and urine, and human plasma. No interferences from endogenous substances were found.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/08/20 alle ore 22:13:46