Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Monkeys of the rainforest in French Guiana are natural reservoirs for P-brasilianum/P-malariae malaria
Autore:
Fandeur, T; Volney, B; Peneau, C; De Thoisy, B;
Indirizzi:
Inst Pasteur Guyane, Mol Parasitol Lab, Cayenne 97306, French Guiana Inst Pasteur Guyane Cayenne French Guiana 97306 nne 97306, French Guiana Hop Andre Bouron, Biol Lab, St Laurent du Maroni 97300, French Guiana Hop Andre Bouron St Laurent du Maroni French Guiana 97300 French Guiana EDF, CNEH, Programme Faune Sauvage, F-73373 Le Bourget du Lac, France EDFLe Bourget du Lac France F-73373 , F-73373 Le Bourget du Lac, France
Titolo Testata:
PARASITOLOGY
, volume: 120, anno: 2000,
parte:, 1
pagine: 11 - 21
SICI:
0031-1820(200001)120:<11:MOTRIF>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
RIBOSOMAL-RNA GENE; PLASMODIUM-MALARIAE; FALCIPARUM; PARASITES; SEQUENCES; BRAZIL; CHINA; VIVAX;
Keywords:
Plasmodium brasilianum; Plasmodium malariae; small subunit rRNA gene; merozoite surface protein-1 gene; monkeys; French Guiana;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Fandeur, T Inst Pasteur Guyane, Mol Parasitol Lab, BP 6010, Cayenne 97306,French Guiana Inst Pasteur Guyane BP 6010 Cayenne French Guiana 97306 Guiana
Citazione:
T. Fandeur et al., "Monkeys of the rainforest in French Guiana are natural reservoirs for P-brasilianum/P-malariae malaria", PARASITOL, 120, 2000, pp. 11-21

Abstract

Monkey blood samples were collected from 214 monkeys relocated as part of the wildlife rescue organized in French Guiana during the filling of the Petit Saut Dam on the Sinnamary River. These samples were rested for malaria parasites by microscopy of thick blood films and by nested PCR for small subunit rRNA genes (SSUrRNA). Parasitic blood forms similar to Plasmodium brasilianum were detected in 4 monkey species: Alouatta seniculus macconnelli,Saguinus midas midas, Pithecia pithecia and Ateles paniscus paniscus, withthe highest prevalence in Alouatta monkeys. PCR was more sensitive than the conventional method for detecting low-grade parasitaemia in positive monkeys. The examination of blood films indicated that 5.6% of the animals carried parasites whereas the nested PCR for ribosomal DNA indicated a prevalence of 11.3%. The P. brasilianum SSUrRNA gene sequence was analysed and aligned with those from P. malariae, P. falciparum and P. vivax. This suggestedthat P. brasilianum and P. malariae are very closely related. Similar results were obtained from analysis of the sequences in P. malariae and P. brasilianum isolates of a polymorphic gene fragment analogous to the merozoite surface protein-1 (MSP-1) gene of P. falciparum. The P. brasilianum/P. malariae sequences were more similar to those of P. vivax than to those of P. falciparum, at least in the gene region examined. The high degree of DNA homology in the sequences of the SSUrRNA and msp1-like genes is consistent with other characterizations demonstrating a taxonomic relationship between P.brasilianum and P. malariae species. Our results provide further evidence that P. brasilianum and P. malariae are virtually identical and should probably be considered to be a single malaria species. This raises the questionas to whether monkeys living in the rainforest are natural reservoirs for both simian and human malaria. These results have implications for the interpretation of the current epidemiological situation in French Guiana.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 14:58:11