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Titolo:
The alpha isoform of protein kinase C is involved in signaling the response of desmosomes to wounding in cultured epithelial cells
Autore:
Wallis, S; Lloyd, S; Wise, I; Ireland, G; Fleming, TP; Garrod, D;
Indirizzi:
Univ Manchester, Sch Biol Sci, Manchester M13 9PT, Lancs, England Univ Manchester Manchester Lancs England M13 9PT M13 9PT, Lancs, England Univ Southampton, Sch Biol Sci, Div Cell Sci, Southampton SO16 7PX, Hants,England Univ Southampton Southampton Hants England SO16 7PX 16 7PX, Hants,England
Titolo Testata:
MOLECULAR BIOLOGY OF THE CELL
fascicolo: 3, volume: 11, anno: 2000,
pagine: 1077 - 1092
SICI:
1059-1524(200003)11:3<1077:TAIOPK>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
KERATIN INTERMEDIATE FILAMENTS; INTERCELLULAR-ADHESION; CALCIUM-CONCENTRATION; EMBRYONIC HEART; CARCINOMA-CELLS; GROWTH-FACTOR; PHOSPHORYLATION; REORGANIZATION; IDENTIFICATION; PLAKOGLOBIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
74
Recensione:
Indirizzi per estratti:
Indirizzo: Garrod, D Univ Manchester, Sch Biol Sci, Manchester M13 9PT, Lancs, England Univ Manchester Manchester Lancs England M13 9PT Lancs, England
Citazione:
S. Wallis et al., "The alpha isoform of protein kinase C is involved in signaling the response of desmosomes to wounding in cultured epithelial cells", MOL BIOL CE, 11(3), 2000, pp. 1077-1092

Abstract

Initiation of reepithelialization upon wounding is still poorly understood. To enhance this understanding, we focus here on changes in the adhesive state of desmosomes of cultured Madin-Darby canine kidney cells in response to wounding of confluent cell sheets. Previous results show that desmosomaladhesion in Madin-Darby canine kidney cells changes from a calcium-dependent state to calcium independence in confluent cell sheets. We show that this change, which requires culture confluence to develop, is rapidly reversedupon wounding of confluent cell sheets. Moreover, the change to calcium dependence in wound edge cells is propagated to cells hundreds of micrometersaway from the wound edge. Rapid transition from calcium independence to calcium dependence also occurs when cells are treated with phorbol esters that activate PKC. PKC inhibitors, including the conventional isoform inhibitor Go6976, cause rapid transition from calcium dependence to calcium independence, even in subconfluent cells. The cellular location of the a isoform of PKC correlates with the calcium dependence of desmosomes. Upon monolayer wounding, PKC alpha translocates rapidly to the cell periphery, becomes Triton X-100 insoluble, and also becomes concentrated in lamellipodia. The PKCalpha a translocation upon wounding precedes both the increase in PKC activity in the membrane fraction and the reversion of desmosomes to calcium dependence. Specific depletion of PKC alpha with an antisense oligonucleotideincreases the number of cells with calcium-independent desmosomes. These results show that PKC alpha participates in a novel signaling pathway that modulates desmosomal adhesion in response to wounding.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/10/20 alle ore 05:33:45