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Titolo:
Stability of insulin-like growth factor I in the gastrointestinal lumen inneonatal pigs
Autore:
Shen, WH; Xu, RJ;
Indirizzi:
Univ Hong Kong, Dept Zool, Hong Kong, Hong Kong, Peoples R China Univ HongKong Hong Kong Hong Kong Peoples R China Kong, Peoples R China
Titolo Testata:
JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION
fascicolo: 3, volume: 30, anno: 2000,
pagine: 299 - 304
SICI:
0277-2116(200003)30:3<299:SOIGFI>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTESTINAL DISACCHARIDASE ACTIVITY; IGF-I; SUCKLING RATS; MILK; FORMULA; PIGLETS; TRACT; AGE;
Keywords:
colostrum; gastrointestinal tract; insulin-like growth factor-I; stability; suckling pigs;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Xu, RJ Univ Hong Kong, Dept Zool, Pokfulam Rd, Hong Kong, Hong Kong, Peoples R China Univ Hong Kong Pokfulam Rd Hong Kong Hong Kong Peoples R China ina
Citazione:
W.H. Shen e R.J. Xu, "Stability of insulin-like growth factor I in the gastrointestinal lumen inneonatal pigs", J PED GASTR, 30(3), 2000, pp. 299-304

Abstract

Background: Insulin-like growth factor (ICF)-I is present in the milk of various species. A prerequisite for any biological activity of milk-borne IGF-I in the suckling young is to survive the gastrointestinal luminal digestion. In the present study, the stability of IGF-I was examined in the gastrointestinal lumen in neonatal pigs. Methods: Iodine-labeled IGF-I was incubated in the gastrointestinal luminal fluids of 3-day-old suckling acid 45-day-old weaned pigs at 37 degrees C for 20 minutes. Degradation of the peptide was analyzed by trichloroacetic acid (TCA) precipitation, liquid chromatography, and receptor binding assay. Results: IGF-I remained unchanged in the gastric fluids of suckling and weaned pigs when determined by TCA precipitation. IGF-I degraded 3%, 18%, and37% in the luminal fluids of the proximal, mid and distal small intestine in suckling piglets compared with 53%, 62%, and 54% in weaned pigs. The results were supported by the chromatography and receptor binding analysis. Porcine colostrum had a capacity to protect ICF-I from gastrointestinal luminal digestion in weaned pigs. Conclusion: Milk-borne IGF-I is stable in the gastrointestinal lumen in suckling pigs and may play a role in regulating postnatal development in the suckling young. (C) 2000 Lippincott Williams & Wilkins, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 12:32:18