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Titolo:
Transduction of human IL-9 receptor cDNA into TF1 cells induces IL-9 dependency and erythroid differentiation
Autore:
Xiao, M; Luo, ZM; Mantel, C; Broxmeyer, HE; Lu, L;
Indirizzi:
Indiana Univ, Sch Med, Walther Oncol Ctr, Indianapolis, IN 46202 USA Indiana Univ Indianapolis IN USA 46202 ol Ctr, Indianapolis, IN 46202 USA Indiana Univ, Sch Med, Dept Med Hematol Oncol, Indianapolis, IN 46202 USA Indiana Univ Indianapolis IN USA 46202 Oncol, Indianapolis, IN 46202 USA Indiana Univ, Sch Med, Dept Microbiol & Immunol, Indianapolis, IN 46202 USA Indiana Univ Indianapolis IN USA 46202 mmunol, Indianapolis, IN 46202 USA Walther Canc Inst, Indianapolis, IN 46202 USA Walther Canc Inst Indianapolis IN USA 46202 t, Indianapolis, IN 46202 USA
Titolo Testata:
JOURNAL OF HEMATOTHERAPY & STEM CELL RESEARCH
fascicolo: 1, volume: 9, anno: 2000,
pagine: 77 - 82
SICI:
1525-8165(200002)9:1<77:TOHIRC>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY FORMATION; CORD-BLOOD; INTERLEUKIN-9; ERYTHROPOIETIN; PROLIFERATION; PROGENITORS; LINE; GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
12
Recensione:
Indirizzi per estratti:
Indirizzo: Lu, L Indiana Univ, Sch Med, Walther Oncol Ctr, 1044 W Walnut St,Room 302,Indianapolis, IN 46202 USA Indiana Univ 1044 W Walnut St,Room 302 Indianapolis IN USA 46202 USA
Citazione:
M. Xiao et al., "Transduction of human IL-9 receptor cDNA into TF1 cells induces IL-9 dependency and erythroid differentiation", J HEMATH ST, 9(1), 2000, pp. 77-82

Abstract

Human growth factor-dependent cell line TF1, which lacks interleukin (IL)-9 receptors (R) and does not grow in IL-9, was transduced with a retroviralvector containing human IL-9R cDNA and a selection marker. An IL-9-dependent TF1 cell line, which could also grow in other cytokines, was establishedafter selection in G418 and could produce mature RBC in response to cytokine stimulation. TF1 cells transduced with the same viral vector without theIL-9R insert cDNA (mock control) and then selected responded the same as nontransduced TF1 cells. They failed to grow in response to IL-9 and did notgenerate RBC. An increased number and size of burst-forming units-erythroid (BFU-E)-like colonies were detected from IL-9R-transduced TF1 cells, compared with mock-transduced cells, in response to erythropoietin (EPO) and IL-9. To evaluate self-renewal and differentiation capacity, colony-replatingassays were performed in the presence of IL-3, GM-CSF, IL-9, and EPO. After four replatings, the cloning efficiency of IL-9R-transduced TF1 cells decreased from 98% to 38%, most likely due to terminal erythroid cell differentiation. In contrast, no change in replating efficiency was detected in mock-transduced cells. TF1 cells stably expressing IL-9R and responding to IL-9 can serve as a cell line model to study the intracellular signals mediating IL-9-induced erythroid cell proliferation and differentiation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 13:15:39