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Titolo:
Substitution of H2O for formamide in the sample preparation protocol for STR analysis using the capillary electrophoresis system: The effects on precision, resolution, and capillary life
Autore:
Biega, LA; Duceman, BW;
Indirizzi:
New York State Police Forens Invest Ctr, Albany, NY 12226 USA New York State Police Forens Invest Ctr Albany NY USA 12226 NY 12226 USA
Titolo Testata:
JOURNAL OF FORENSIC SCIENCES
fascicolo: 5, volume: 44, anno: 1999,
pagine: 1029 - 1031
SICI:
0022-1198(199909)44:5<1029:SOHFFI>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
TETRANUCLEOTIDE REPEAT POLYMORPHISM; TESTICULAR CANCER; DIMETHYLFORMAMIDE; GENE; AMELOGENIN; LOCUS; PCR;
Keywords:
forensic science; short tandem repeats; DNA typing; capillary electrophoresis; resolution D3S1358; vWA; FGA; amelogenin; THO1; TPOX; CSF1PO; D5S818; D13S317; D7S820;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Biega, LA New York State Police Forens Invest Ctr, Bldg 30,State Campus,1220 Washington Ave, Albany, NY 12226 USA New York State Police Forens InvestCtr Bldg 30,State Campus,1220 Washington Ave Albany NY USA 12226
Citazione:
L.A. Biega e B.W. Duceman, "Substitution of H2O for formamide in the sample preparation protocol for STR analysis using the capillary electrophoresis system: The effects on precision, resolution, and capillary life", J FOREN SCI, 44(5), 1999, pp. 1029-1031

Abstract

Water has been evaluated as a suitable solvent to replace formamide in thepreparation of samples For capillary electrophoresis analysis. Critical aspects relevant to forensic analysis were studied to test the validity of the substitution. Correct assignment of allele labels was reproducibly performed on known samples, and size determination was found to be precise. Threehundred injections, regardless the method of preparation, may be run on a single capillary without loss of precision or resolution.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 06:30:36