Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
TWGDAM validation of a nine-locus and a four-locus fluorescent STR multiplex system
Autore:
Micka, KA; Amiott, EA; Hockenberry, TL; Sprecher, CJ; Lins, AM; Rabbach, DR; Taylor, JA; Bacher, JW; Glidewell, DE; Gibson, SD; Crouse, CA; Schumm, JW;
Indirizzi:
Promega Corp, Madison, WI 53711 USA Promega Corp Madison WI USA 53711Promega Corp, Madison, WI 53711 USA Palm Beach Cty Sheriffs Off, Crime Lab, W Palm Beach, FL USA Palm Beach Cty Sheriffs Off W Palm Beach FL USA ab, W Palm Beach, FL USA
Titolo Testata:
JOURNAL OF FORENSIC SCIENCES
fascicolo: 6, volume: 44, anno: 1999,
pagine: 1243 - 1257
SICI:
0022-1198(199911)44:6<1243:TVOANA>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
TANDEM REPEAT LOCI; TAQ DNA-POLYMERASE; ALLELIC LADDER; IDENTIFICATION; GENE; VWA;
Keywords:
forensic science; short tandem repeat; polymerase chain reaction; validation; TWGDAM; PowerPlex; FFFL; microsatellite; polymorphism; D16S539; D7S820; D13S317; D5SA818; CSF1PO; TPOX; THO1; VWA; amelogenin; F13A01; FESFPS; F13B; LPL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Amiott, EA Promega Corp, 2800 Woods Hollow Rd, Madison, WI 53711 USA Promega Corp 2800 Woods Hollow Rd Madison WI USA 53711 3711 USA
Citazione:
K.A. Micka et al., "TWGDAM validation of a nine-locus and a four-locus fluorescent STR multiplex system", J FOREN SCI, 44(6), 1999, pp. 1243-1257

Abstract

The Gene Print(R) PowerPlex(TM) 1.1/Amelogenin and FFFL Fluorescent STR Systems have been validated following the recommendations presented by the Technical Working Group on DNA Analysis Methods (TWGDAM). The PowerPlex(TM) 1.1/Amelogenin System supports simultaneous amplification of eight short tandem repeat loci and the Amelogenin gender identification marker. The loci D16S539, D7S820, D13S317, and D5S818 are labeled with fluorescein (FL) whilethe loci CSF1PO, TP0X, TH01, vWA and Amelogenin are labeled with carboxy-tetramethylrhodamine (TMR). The FFFL Multiplex System is composed of the loci F13A01, FESFPS, F13B, and LPL, each labeled with fluorescein. We have observed no overlap of alleles across loci labeled with an individual fluorescent dye. Samples of each system were amplified and labeled in a single reaction, separated by electrophoresis through a denaturing polyacrylamide gel,and amplified alleles detected using a Hitachi FMBIO(R) Fluorescent Scanner. Alterations from the standard amplification protocols in cycle number and annealing temperature generally produced excellent results. In experiments testing sensitivity as little as 0.2 ng of DNA template could be detected. As expected, different body fluids from the same individuals generated identical DNA profile results. Template DNA derived from blood-strains deposited on a variety of matrix supports displayed robust amplification except for material derived from deposits on wood and Japanese orchid leaves. Mixtures of DNA templates could be interpreted with the minor component present in as little as ten percent of the total sample. Monoplex and multiplex amplifications produced identical amplified allele patterns, indicating that STR multiplex systems save template and increase efficiency in the amplification procedure without loss of quality. Analyses of genotype frequencies inAfrican-American, Caucasian-American and Hispanic-American populations using all twelve loci were used to determine matching probabilities smaller than 1 in 1.14 x 10(8) and 1 in 2658 for the PowerPlex(TM) 1.1 and the FFFL Multiplex Systems, respectively. The matching probability achieved with the two systems combined is smaller than 1 in 3.03 x 10(11). The independence of alleles within loci was generally demonstrated by applying the exact testto demonstrate Hardy-Weinberg Equilibrium. All of the studies performed indicate that the PowerPlex(TM) 1.1/Amelogenin and FFFL Multiplex Systems arepowerful, robust, and reliable investigative tools that can be used in theanalysis of forensic samples.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 22:50:49