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Titolo:
Cloning and characterization of a new isoform of skeletal muscle triadin
Autore:
Marty, I; Thevenon, D; Scotto, C; Groh, S; Sainnier, S; Robert, M; Grunwald, D; Villaz, M;
Indirizzi:
Commissariat Energie Atom, INSERM, E9931, Dept Biol Mol & Struct Canaux Ion & Signalisat, F-38054 Grenoble, France Commissariat Energie Atom Grenoble France F-38054 38054 Grenoble, France
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 11, volume: 275, anno: 2000,
pagine: 8206 - 8212
SICI:
0021-9258(20000317)275:11<8206:CACOAN>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
JUNCTIONAL SARCOPLASMIC-RETICULUM; RYANODINE RECEPTOR; DIHYDROPYRIDINE RECEPTOR; FOOT PROTEIN; BIOCHEMICAL-CHARACTERIZATION; GLYCOPROTEIN TRIADIN; CALSEQUESTRIN; LOCALIZATION; COMPLEX; PURIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Marty, I Commissariat Energie Atom, INSERM, E9931, Dept Biol Mol & Struct Canaux Ion & Signalisat, 17 Rue Martyrs, F-38054 Grenoble, France Commissariat Energie Atom 17 Rue Martyrs Grenoble France F-38054
Citazione:
I. Marty et al., "Cloning and characterization of a new isoform of skeletal muscle triadin", J BIOL CHEM, 275(11), 2000, pp. 8206-8212

Abstract

We have shown that several isoforms of triadin, a protein involved in calcium release process through the ryanodine receptor, are expressed in rat skeletal muscle, and we have cloned two of these isoforms. One is the rat homolog of the 95-kDa triadin identified in rabbit skeletal muscle, and the second one, shorter, is a truncated form of the previous one, but with a new unique COOH-terminal end. We propose to name the two proteins identified here Trisk 95 and Trisk 51. We have produced antibodies specific to each isoform. Using these antibodies, we have shown that the newly identified protein, Trisk 51, is actually expressed in adult rat skeletal muscle and also inrat embryo skeletal muscle. Immunofluorescent labeling of rat skeletal muscle with anti-Trisk 95, anti-Trisk 51, or anti-ryanodine receptor antibodies shows a similar localization of these proteins, in the tissue. Transfection of L6 cells with cDNA of Trisk 51 or Trisk 95 leads to the expression ofproteins with the expected molecular weight, identical to those detected in rat skeletal muscle. Both proteins appear during differentiation of satellite cells in myotubes which may indicate the involvement of these two isoforms in the building of a functional calcium, release machinery.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 13:38:42