Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Specificity of DNA lesion bypass by the yeast DNA polymerase eta
Autore:
Yuan, FH; Zhang, YB; Rajpal, DK; Wu, XH; Guo, DY; Wang, M; Taylor, JS; Wang, ZG;
Indirizzi:
Univ Kentucky, Grad Ctr Toxicol, Lexington, KY 40536 USA Univ Kentucky Lexington KY USA 40536 Ctr Toxicol, Lexington, KY 40536 USA Washington Univ, Dept Chem, St Louis, MO 63130 USA Washington Univ St Louis MO USA 63130 , Dept Chem, St Louis, MO 63130 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 11, volume: 275, anno: 2000,
pagine: 8233 - 8239
SICI:
0021-9258(20000317)275:11<8233:SODLBB>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
SACCHAROMYCES-CEREVISIAE GENE; ESCHERICHIA-COLI DINB; INDUCED MUTAGENESIS; HOMOLOG; PROTEIN; ENCODES; SEQUENCE; BINDING; DOMAINS; REV3;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Wang, ZG Univ Kentucky, Grad Ctr Toxicol, 306 Hlth Sci Res Bldg, Lexington, KY 40536 USA Univ Kentucky 306 Hlth Sci Res Bldg Lexington KY USA 40536 36 USA
Citazione:
F.H. Yuan et al., "Specificity of DNA lesion bypass by the yeast DNA polymerase eta", J BIOL CHEM, 275(11), 2000, pp. 8233-8239

Abstract

DNA polymerase eta (Pol eta, xeroderma pigmentosum variant, or Rad30) plays an important role in an error-free response to unrepaired UV damage during replication. It faithfully synthesizes DNA opposite a thymine-thymine cis-syn-cyclobutane dimer. We have purified the yeast Pol eta and studied its lesion bypass activity in vitro with various types of DNA damage. The yeastPol eta lacked a nuclease or a proofreading activity. It efficiently bypassed 8-oxoguanine, incorporating C, A, and G opposite the lesion with a relative efficiency of similar to 100: 56:14, respectively. The yeast Pol eta efficiently incorporated a C opposite an acetylaminofluorene-modified G, andefficiently inserted a G or less frequently an A opposite an apurinic/apyrimidinic (AP) site but was unable to extend the DNA synthesis further in both cases. However, some continued DNA synthesis was observed in the presence of the yeast Pol zeta following the Pol eta action opposite an AP site, achieving true lesion bypass. In contrast, the yeast polo was able to bypassefficiently a template AP site, predominantly incorporating an A residue opposite the lesion, These results suggest that other than UV damage, Pol eta may also play a role in bypassing additional DNA lesions, some of which can be error-prone.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/07/20 alle ore 08:48:40