Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
A protein conjugation system in yeast with homology to biosynthetic enzymereaction of prokaryotes
Autore:
Furukawa, K; Mizushima, N; Noda, T; Ohsumi, Y;
Indirizzi:
Natl Inst Basic Biol, Dept Cell Biol, Okazaki, Aichi 4448585, Japan Natl Inst Basic Biol Okazaki Aichi Japan 4448585 ki, Aichi 4448585, Japan Japan Sci & Technol Corp, PRESTO, Okazaki, Aichi 444, Japan Japan Sci & Technol Corp Okazaki Aichi Japan 444 kazaki, Aichi 444, Japan Suntory Inst Med Res & Dev, Gunma 3700503, Japan Suntory Inst Med Res & Dev Gunma Japan 3700503 Dev, Gunma 3700503, Japan
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 11, volume: 275, anno: 2000,
pagine: 7462 - 7465
SICI:
0021-9258(20000317)275:11<7462:APCSIY>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
UBIQUITIN-ACTIVATING ENZYME; SACCHAROMYCES-CEREVISIAE; ESCHERICHIA-COLI; KINASE-C; GENE; MOLYBDOPTERIN; SMT3P;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Ohsumi, Y Natl Inst Basic Biol, Dept Cell Biol, 38 Nishigonaka, Okazaki, Aichi 4448585, Japan Natl Inst Basic Biol 38 Nishigonaka Okazaki Aichi Japan4448585
Citazione:
K. Furukawa et al., "A protein conjugation system in yeast with homology to biosynthetic enzymereaction of prokaryotes", J BIOL CHEM, 275(11), 2000, pp. 7462-7465

Abstract

Protein conjugation, such as ubiquitination, is the process by which the C-terminal glycine of a small modifier protein is covalently attached to target protein(s) through sequential reactions with an activating enzyme and conjugating enzymes. Here we report on a novel protein conjugation system inyeast. A newly identified ubiquitin related modifier, Urm1 is a 99-amino acid protein terminated with glycine-glycine. Urm1 is conjugated to target proteins, which requires the C-terminal glycine of Urm1. At the first step of this reaction, Urm1 forms a thioester with a novel E1-like protein, Uba4. Delta urm1 and Delta uba4 cells showed a temperature-sensitive growth phenotype. Urm1 and Uba4 show similarity to prokaryotic proteins essential for molybdopterin and thiamin biosynthesis, although the Urm1 system is not involved in these pathways. This is the fifth conjugation system in yeast, following ubiquitin, Smt3, Rub1, and Apg12, but it is unique in respect to relation to prokaryotic enzyme systems. This fact may provide an important clue regarding evolution of protein conjugation systems in eukaryotic cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 17/01/21 alle ore 15:45:09