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Titolo:
A 90-kilobase conjugative chromosomal element coding for biphenyl and salicylate catabolism in Pseudomonas putida KF715
Autore:
Nishi, A; Tominaga, K; Furukawa, K;
Indirizzi:
Kyushu Univ, Grad Sch, Div Bioresource & Bioenvironm Sci, Fukuoka 8128581,Japan Kyushu Univ Fukuoka Japan 8128581 Bioenvironm Sci, Fukuoka 8128581,Japan
Titolo Testata:
JOURNAL OF BACTERIOLOGY
fascicolo: 7, volume: 182, anno: 2000,
pagine: 1949 - 1955
SICI:
0021-9193(200004)182:7<1949:A9CCEC>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
SP. STRAIN B13; ENCODING BIPHENYL; GENE-CLUSTER; STREPTOCOCCUS-FAECALIS; NUCLEOTIDE-SEQUENCE; DEGRADATION GENES; HYDROXYLASE GENE; SOIL BACTERIA; BPH OPERON; TRANSPOSON;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Furukawa, K Kyushu Univ, Grad Sch, Div Bioresource & Bioenvironm Sci, Fukuoka 8128581,Japan Kyushu Univ Fukuoka Japan 8128581 Sci, Fukuoka 8128581,Japan
Citazione:
A. Nishi et al., "A 90-kilobase conjugative chromosomal element coding for biphenyl and salicylate catabolism in Pseudomonas putida KF715", J BACT, 182(7), 2000, pp. 1949-1955

Abstract

The biphenyl and salicylate metabolic pathways in Pseudomonas putida KP715are chromosomally encoded. The bph gene cluster coding for the conversion of biphenyl to benzoic acid and the sal gene cluster coding for the salicylate meta-pathway were obtained, from the KF715 genomic cosmid libraries. These two gene clusters were separated by 10-kb DNA and were highly prone to deletion when KF715 was grown in nutrient medium. Two types of deletions took place at the region including only the bph genes (ca. 40 kb) or at the region including both the bph and sal genes (ca. 70 kb). A 90-kb, DNA region, including both the bph and sal genes (termed the bph-sal element), was transferred by conjugation from KP715 to P. putida AC30. Such transconjugantsgained the ability to grow on biphenyl and salicylate as the sole sources of carbon. The bph and sal element was located on the chromosome of the recipient. The bph.-sal element in strain AC30 was also highly prone to deletion; however, it could be mobilized to the chromosome of P. putida KT2440 and the two deletion mutants of KF715.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/01/21 alle ore 04:02:07