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Titolo:
Expression of the multiple antibiotic resistance operon (mar) during growth of Escherichia coli as a biofilm
Autore:
Maira-Litran, T; Allison, DG; Gilbert, P;
Indirizzi:
Univ Manchester, Sch Pharm & Pharmaceut Sci, Manchester M13 9PL, Lancs, England Univ Manchester Manchester Lancs England M13 9PL M13 9PL, Lancs, England
Titolo Testata:
JOURNAL OF APPLIED MICROBIOLOGY
fascicolo: 2, volume: 88, anno: 2000,
pagine: 243 - 247
SICI:
1364-5072(200002)88:2<243:EOTMAR>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
ORGANIC-SOLVENT TOLERANCE; OXIDATIVE STRESS GENES; BACTERIAL BIOFILMS; TETRACYCLINE; CHLORAMPHENICOL; OVEREXPRESSION; ACTIVATION; REPRESSOR; MUTATIONS; INDUCTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Maira-Litran, T Univ Complutense Madrid, Dept Nutr & Bromatol 111, Avd Puerta de Hierro, E-28040 Madrid, Spain Univ Complutense Madrid Avd Puerta de Hierro Madrid Spain E-28040
Citazione:
T. Maira-Litran et al., "Expression of the multiple antibiotic resistance operon (mar) during growth of Escherichia coli as a biofilm", J APPL MICR, 88(2), 2000, pp. 243-247

Abstract

The multiple antibiotic resistance (mar) operon is a global regulator controlling the expression of various genes in Escherichia coli which constitutes the mar regulon. Upregulation of mar leads to a multi-drug resistant phenotype, which includes resistance towards structurally unrelated antibiotics, organic solvents and the disinfectant pine oil. Biofilms also display similar decreases in susceptibility to antimicrobial agents. A marO(II)-lacZ fusion strain (SPC105) of E. coli was used to monitor mar expression under various growth conditions including batch, continuous and biofilm culture. In chemically-defined media (CDM), mar expression was maximal in mid-log and declined in the stationary phase. Conversely, in rich media (Luria-Bertani broth), minimal expression in mid-log was followed by an increase in the stationary phase. In continuous culture, expression was inversely related to specific growth rate (mu = 0.05-0.4 h(-1)). LacZ expression by the marO(II)-lacZ fusion was generally low within the total biofilm population and equivalent to that of stationary phase cultures grown in batch culture. When the expression of mar in CDM batch culture was compared with that in biofilm populations, beta-galactosidase activity was generally higher throughout batch culture than in the attached population. Overall, these results suggest that while mar expression will be greatest within the depths of a biofilm where growth rates are suppressed, its probable induction within biofilmscannot explain the elevated levels of antibiotic resistance observed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 10:42:27