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Titolo:
Radiation retards muscle differentiation but does not affect osteoblastic differentiation induced by bone morphogenetic protein-2 in C2C12 myoblasts
Autore:
Ikeda, S; Hachisu, R; Yamaguchi, A; Gao, YH; Okano, T;
Indirizzi:
Showa Univ, Sch Dent, Dept Radiol, Ohta Ku, Tokyo 1458515, Japan Showa Univ Tokyo Japan 1458515 ept Radiol, Ohta Ku, Tokyo 1458515, Japan Nagasaki Univ, Sch Dent, Dept Oral Pathol, Nagasaki 8528588, Japan Nagasaki Univ Nagasaki Japan 8528588 ral Pathol, Nagasaki 8528588, Japan
Titolo Testata:
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
fascicolo: 3, volume: 76, anno: 2000,
pagine: 403 - 411
SICI:
0955-3002(200003)76:3<403:RRMDBD>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSFORMING-GROWTH-FACTOR; IONIZING-RADIATION; MYOD FAMILY; CELLS; ACTIVATION; INHIBITOR; MEMBRANE; RECEPTOR; LINEAGE; INVITRO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Hachisu, R Showa Univ, Sch Dent, Dept Radiol, Ohta Ku, 2-1-1 Kitasenzoku, Tokyo 1458515, Japan Showa Univ 2-1-1 Kitasenzoku Tokyo Japan 1458515 458515, Japan
Citazione:
S. Ikeda et al., "Radiation retards muscle differentiation but does not affect osteoblastic differentiation induced by bone morphogenetic protein-2 in C2C12 myoblasts", INT J RAD B, 76(3), 2000, pp. 403-411

Abstract

Purpose: Effects of radiation on the differentiation of C2C12 myoblasts were studied using cell cultures in which the cells differentiated into either muscle cells or osteoblasts. Materials and methods: A mouse skeletal myoblast cell line, C2C12, was cultured and irradiated at confluence. Muscle differentiation was estimated bycounting the number of myosin heavy chain (MHC)-positive multinucleated myotubes. Osteoblast differentiation was analysed by measuring alkaline phosphatase (ALP) activity and osteocalcin production in C2C12 cells treated with recombinant human bone morphogenetic protein-2 (rhBMP-2). Results: Myotube formation in C2C12 cells was inhibited by 2 and 4Gy irradiation, but recovered to non-irradiated control levels following further culture. In irradiated cultures, the number of MHC-positive mononucleated cells increased significantly. Irradiation did not, however, alter the induction of osteoblastic phenotypes induced by rhBMP-2 in C2C12. Irradiation alsodid not affect the expression of MyoD mRNA and myogenin mRNA in myogenic differentiation and induction of osteocalcin mRNA in osteoblastic differentiation. Conclusions: During differentiation of C2C12 cells, myotube formation was delayed after irradiation, while induction of osteoblasts with BMP-2 was unaffected.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 10:25:19