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Titolo:
Spatial expression patterns of epidermal growth factor receptor gene transcripts in the postnatal mammalian cochlea
Autore:
Zine, A; Nyffeler, M; de Ribaupierre, F;
Indirizzi:
Univ Lausanne, Inst Physiol, CH-1005 Lausanne, Switzerland Univ Lausanne Lausanne Switzerland CH-1005 CH-1005 Lausanne, Switzerland
Titolo Testata:
HEARING RESEARCH
fascicolo: 1-2, volume: 141, anno: 2000,
pagine: 19 - 27
SICI:
0378-5955(200003)141:1-2<19:SEPOEG>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-SITU HYBRIDIZATION; AUDITORY HAIR-CELLS; FACTOR-ALPHA; RAT-BRAIN; SENSORY EPITHELIA; MESSENGER-RNA; PROLIFERATION; FIBROBLAST; NEURONS; PROBES;
Keywords:
reverse transcriptase-polymerase chain reaction; organ of Corti; epidermal growth factor receptor; in situ hybridization; rat;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: de Ribaupierre, F Univ Lausanne, Inst Physiol, 7 Rue Bugnon, CH-1005 Lausanne, Switzerland Univ Lausanne 7 Rue Bugnon Lausanne Switzerland CH-1005
Citazione:
A. Zine et al., "Spatial expression patterns of epidermal growth factor receptor gene transcripts in the postnatal mammalian cochlea", HEARING RES, 141(1-2), 2000, pp. 19-27

Abstract

Recent in vitro studies demonstrated that members of the epidermal growth factor (EGF) family are involved in hair cell replacement in the postnatal mammalian organ of Corti (OC) after ototoxic damage. This suggests a role for the EGF receptor (EGFR) in this process. We examined the expression of EGFR mRNA within the normal postnatal day 3 (P3) and adult rat cochlear epithelium by RT-PCR and examined its cellular localization with non-radioactive in situ hybridization in P3 and adult cochleae. RT-PCR demonstrated that EGFR mRNA is expressed in P3 and adult cochlear epithelium. In situ hybridization localized high levels of EGFR transcripts in the OC, spiral ganglion, Kolliker's organ and detectable levels in the supporting cells and the stria vascularis of P3 cochlea. In the adult cochlea, EGFR transcripts were detected only in the spiral ganglion. Our results support that the EGFR is implicated in the differentiation of several cochlear cell types and in the response of OC to ototoxic damage of the P3 rat. In the adult, it may participate in the maintenance of the mature neurons and its absence in the OC may contribute to the lack of regenerative responses in the adult cochlea. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 14:30:31