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Titolo:
Autocrine expression and ontogenetic functions of the PACAP ligand/receptor system during sympathetic development
Autore:
DiCicco-Bloom, E; Deutsch, PJ; Maltzman, J; Zhang, JW; Pintar, JE; Zheng, J; Friedman, WF; Zhou, XF; Zaremba, T;
Indirizzi:
Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Dept Neurosci & Cell Biol, Piscataway, NJ 08854 USA Univ Med & Dent New Jersey Piscataway NJ USA 08854 scataway, NJ 08854 USA Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Dept Pediat, New Brunswick, NJ 08901 USA Univ Med & Dent New Jersey New Brunswick NJ USA 08901 swick, NJ 08901 USA Cornell Univ, Weill Med Coll, Div Mol Med, New York, NY 10021 USA Cornell Univ New York NY USA 10021 l, Div Mol Med, New York, NY 10021 USA Columbia Univ Coll Phys & Surg, Dept Pathol, New York, NY 10032 USA Columbia Univ Coll Phys & Surg New York NY USA 10032 w York, NY 10032 USA
Titolo Testata:
DEVELOPMENTAL BIOLOGY
fascicolo: 2, volume: 219, anno: 2000,
pagine: 197 - 213
SICI:
0012-1606(20000315)219:2<197:AEAOFO>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYCLASE-ACTIVATING POLYPEPTIDE; NERVE GROWTH-FACTOR; VASOACTIVE-INTESTINAL-PEPTIDE; SUPERIOR CERVICAL-GANGLION; 38-AMINO ACID FORM; NEURONS IN-VIVO; ADENYLATE-CYCLASE; PC12 CELLS; NEUROTROPHIC FACTOR; SIGNAL-TRANSDUCTION;
Keywords:
neurogenesis; proliferation; mitosis; neurotrophins; trkA; trkC; VIP; trophic factors; survival; Ca2+ flux; cAMP; phosphatidyl inositol;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
88
Recensione:
Indirizzi per estratti:
Indirizzo: DiCicco-Bloom, E Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Dept Neurosci & Cell Biol, Piscataway, NJ 08854 USA Univ Med & Dent New Jersey Piscataway NJ USA 08854 4 USA
Citazione:
E. DiCicco-Bloom et al., "Autocrine expression and ontogenetic functions of the PACAP ligand/receptor system during sympathetic development", DEVELOP BIO, 219(2), 2000, pp. 197-213

Abstract

The superior cervical ganglion (SCG) is a well-characterized model of neural development, in which several regulatory signals have been identified. Vasoactive intestinal peptide (VIP) has been found to regulate diverse ontogenetic processes in sympathetics, though functional requirements for high peptide concentrations suggest that other ligands are involved. We now describe expression and functions of pituitary adenylate cyclase-activating polypeptide (PACAP) during SCG ontogeny, suggesting that the peptide plays critical roles in neurogenesis. PACAP and PACAP receptor (PAC(1)) mRNA's were detected at embryonic days 14.5 (E14.5) through E17.5 in vivo and virtually all precursors exhibited ligand and receptor, indicating that the system isexpressed as neuroblasts proliferate. Exposure of cultured precursors to PACAP peptides, containing 27 or 38 residues, increased mitogenic activity 4-fold. Significantly, PACAP was 1000-fold more potent than VIP and a highlypotent and selective antagonist entirely blocked effects of micromolar VIP, consistent with both peptides acting via PAC(1) receptors. Moreover, PACAP potently enhanced precursor survival more than 2-fold, suggesting that previously defined VIP effects were mediated via PAC(1) receptors and that PACAP is the more significant developmental signal. In addition to neurogenesis, PACAP promoted neuronal differentiation, increasing neurite outgrowth 4-fold and enhancing expression of neurotrophin receptors trkC and trkA. Since PACAP potently activated cAMP and PI pathways and increased intracellular Ca2+, the peptide may interact with other developmental signals. PACAP stimulation of precursor mitosis, survival, and trk receptor expression suggests that the signaling system plays a critical autocrine role during sympathetic neurogenesis. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 17:41:35