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Titolo:
Lipase activity from strains of Pasteurella multocida
Autore:
Pratt, J; Cooley, JD; Purdy, CW; Straus, DC;
Indirizzi:
Texas Tech Univ, Hlth Sci Ctr, Dept Microbiol & Immunol, Lubbock, TX 79430USA Texas Tech Univ Lubbock TX USA 79430 biol & Immunol, Lubbock, TX 79430USA ARS, Conservat & Prod Res Lab, USDA, Bushland, TX 79012 USA ARS Bushland TX USA 79012 at & Prod Res Lab, USDA, Bushland, TX 79012 USA
Titolo Testata:
CURRENT MICROBIOLOGY
fascicolo: 5, volume: 40, anno: 2000,
pagine: 306 - 309
SICI:
0343-8651(200005)40:5<306:LAFSOP>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEURAMINIDASE; ORIGIN; CELLS; A-3;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Straus, DC Texas Tech Univ, Hlth Sci Ctr, Dept Microbiol & Immunol, Lubbock, TX 79430USA Texas Tech Univ Lubbock TX USA 79430 nol, Lubbock, TX 79430USA
Citazione:
J. Pratt et al., "Lipase activity from strains of Pasteurella multocida", CURR MICROB, 40(5), 2000, pp. 306-309

Abstract

Thirteen clinical isolates of Pasteurella multocida from a variety of different animals and humans were examined for their ability to produce lipase. Lipase substrates used included Tween 20, Tween 40, Tween 80, and Tween 85. Lipase activity was detected in the filtrates of organisms grown to the exponential phase in Roswell Park Memorial Institute-1640 defined media (RPMI-1640), but activity increased in the filtrates when the cultures were allowed to proceed to the stationary phase. All strains examined (except for serotype 2) showed lipase activity against at least one of the Tweens. Tween40 was the best substrate to demonstrate lipase activity. Pasteurella multocida serotype 8 produced the most active lipase against Tween 40 (3,561.7 units of activity/mu g of protein). This activity continued to increase after P. multocida entered a stationary growth phase. P. multocida lipase activity was optimal at pH 8.0. Lipase activity of P. multocida serotype 8 was eluted from a Sepharose 2B column at several points, indicating that several lipases may be produced in vitro by this organism. These data demonstratethat clinical isolates of P. multocida produce lipase; therefore, this enzyme should be considered a potential virulence factors for this organism.

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Documento generato il 26/09/20 alle ore 12:00:36