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Titolo:
An improved HPLC-ED method for monitoring plasma levels of clozapine and its active metabolites in schizophrenic patients
Autore:
Raggi, MA; Bugamelli, F; Sabbioni, C; De Ronchi, D; Pinzauti, S; Volterra, V;
Indirizzi:
Univ Bologna, Dept Pharmaceut Sci, I-40126 Bologna, Italy Univ Bologna Bologna Italy I-40126 harmaceut Sci, I-40126 Bologna, Italy Univ Bologna, Psychiat Clin, I-40123 Bologna, Italy Univ Bologna BolognaItaly I-40123 Psychiat Clin, I-40123 Bologna, Italy Univ Florence, Dept Pharmaceut Sci, I-50121 Florence, Italy Univ FlorenceFlorence Italy I-50121 maceut Sci, I-50121 Florence, Italy
Titolo Testata:
CHROMATOGRAPHIA
fascicolo: 3-4, volume: 51, anno: 2000,
pagine: 147 - 153
SICI:
0009-5893(200002)51:3-4<147:AIHMFM>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; SOLID-PHASE EXTRACTION; ULTRAVIOLET DETECTION; HUMAN-SERUM; MAJOR METABOLITES; N-OXIDE; AMPEROMETRIC DETECTION; GAS-CHROMATOGRAPHY; DESMETHYLCLOZAPINE; NORCLOZAPINE;
Keywords:
column liquid chromatography; amperometric detector; clozapine; clozapine metabolites; plasma levels;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Raggi, MA Univ Bologna, Dept Pharmaceut Sci, Via Belmeloro 6, I-40126 Bologna, Italy Univ Bologna Via Belmeloro 6 Bologna Italy I-40126 logna, Italy
Citazione:
M.A. Raggi et al., "An improved HPLC-ED method for monitoring plasma levels of clozapine and its active metabolites in schizophrenic patients", CHROMATOGR, 51(3-4), 2000, pp. 147-153

Abstract

An HPLC method with electrochemical detection has been developed for the determination of clozapine and its main metabolites, desmethylclozapine and clozapine N-oxide, in human plasma. An accurate pretreatment of the biological samples was implemented by means of solid phase extraction (SPE) on HLBcartridges. This improved pretreatment, together with a new mobile phase, allows for the accurate determination of clozapine N-oxide, which could notbe quantitated by a previous method. The method uses only 100 mu L of plasma for one complete analysis and shows good recovery values for all three analytes. The eluates from the SPE procedure were chromatographed in a reversed phase C18 column using a mobile phase composed of phosphate buffer, acetonitrile and methanol. Clozapine, desmethylclozapine and clozapine N-oxidewere eluted in less than 10 minutes, without any interference from the biological matrix. Linearity was observed over the 2.50 - 150 ng mL(-1) (clozapine and desmethylclozapine) or 1.25 - 75 ng mL(-1) (clozapine N-oxide) range for the three analytes, with satisfactory repeatability values. The limit of detection was 0.3 ng mL(-1) for clozapine and desmethylclozapine, and 0.6 for clozapine N-oxide. The application to plasma samples of patients treated with Leponex gave good results, No interference from other common central nervous system drugs was found. This method seems to be a useful tool for pharmacokinetic studies and for clinical monitoring, because of its need for small plasma samples and its high sensitivity and selectivity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/01/20 alle ore 01:45:37