Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Reversible enzyme immobilization via a very strong and nondistorting ionicadsorption on support-polyethylenimine composites
Autore:
Mateo, C; Abian, O; Fernandez-Lafuente, R; Guisan, JM;
Indirizzi:
CSIC, Inst Catalisis, Dept Biocatalisis, E-28049 Madrid, Spain CSIC Madrid Spain E-28049 isis, Dept Biocatalisis, E-28049 Madrid, Spain
Titolo Testata:
BIOTECHNOLOGY AND BIOENGINEERING
fascicolo: 1, volume: 68, anno: 2000,
pagine: 98 - 105
SICI:
0006-3592(20000405)68:1<98:REIVAV>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
STABILIZATION; POLY(ETHYLENEIMINE); BIOCATALYSTS; AGAROSE; CELLS; GELS;
Keywords:
reversible immobilization of proteins; ionic adsorption of proteins; polyethylenimine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Guisan, JM CSIC, Inst Catalisis, Dept Biocatalisis, Campus Univ Autonoma,Cantoblanco,E-28049 Madrid, Spain CSIC Campus Univ Autonoma,Cantoblanco Madrid Spain E-28049 ain
Citazione:
C. Mateo et al., "Reversible enzyme immobilization via a very strong and nondistorting ionicadsorption on support-polyethylenimine composites", BIOTECH BIO, 68(1), 2000, pp. 98-105

Abstract

New tailor-made anionic exchange resins have been prepared, based an filmsof large polyethylenimine polymers (e.g., MW 25,000) completely coating, via covalent immobilization, the surface of different porous supports (agarose, silica, polymeric resins). Most proteins contained in crude extracts from different sources have been very strongly adsorbed on them. Ionic exchange properties of such composites strongly depend on the size of polyethylenimine polymers as well as on the exact conditions of the covalent coating of the solids with the polymer. On the contrary, similar coating protocols yield similar matrices by using different porous supports as starring material. For example, 77% of all proteins contained in crude extracts from Escherichia coil were adsorbed, at low ionic strength, on the best matrices, andless than 15% of the adsorbed proteins were eluted from the support in thepresence of 0.3 M NaCl. Under these conditions, 100% of the adsorbed proteins were eluted from conventional DEAE supports, Such polyethylenimine-support composites were also very suitable to perform very strong and nondistorting reversible immobilization of industrial enzymes. For example, lipase from Candida rugosa (CRL), beta-galactosidase from Aspergillus oryzae and D-amino acid oxidase (DAAO) from Rhodotorula gracilis, were adsorbed on such matrices in a few minutes at pH 7.0 and 4 degrees C. Immobilized enzymes preserved 100% of catalytic activity and remained fully immobilized in 0.2 M NaCl. In addition to that, CRL and DAAO were highly stabilized upon immobilization, Stabilization of DAAO, a dimeric enzyme, seems to be due to the involvement of both enzyme subunits in the ionic adsorption. (C) 2000 John Wiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 20:46:40