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Titolo:
Reduced expression of the regulatory CD4+T cell subset is related to Th1/Th2 balance and disease severity in rheumatoid arthritis
Autore:
Yudoh, K; Matsuno, H; Nakazawa, F; Yonezawa, T; Kimura, T;
Indirizzi:
Toyama Med & Pharmaceut Univ, Dept Orthopaed Surg, Sugitani, Toyama 9300194, Japan Toyama Med & Pharmaceut Univ Sugitani Toyama Japan 9300194 9300194, Japan
Titolo Testata:
ARTHRITIS AND RHEUMATISM
fascicolo: 3, volume: 43, anno: 2000,
pagine: 617 - 627
SICI:
0004-3591(200003)43:3<617:REOTRC>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
T-CELLS; TELOMERASE ACTIVITY; AUTOIMMUNE-DISEASE; CYTOKINE PATTERNS; INTERLEUKIN-10; MICE; IL-10; RECEPTOR; LYMPHOCYTES; STIMULATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Yudoh, K Toyama Med & Pharmaceut Univ, Dept Orthopaed Surg, 2630 Sugitani,Sugitani, Toyama 9300194, Japan Toyama Med & Pharmaceut Univ 2630 SugitaniSugitani Toyama Japan 9300194
Citazione:
K. Yudoh et al., "Reduced expression of the regulatory CD4+T cell subset is related to Th1/Th2 balance and disease severity in rheumatoid arthritis", ARTH RHEUM, 43(3), 2000, pp. 617-627

Abstract

Objective. To elucidate the involvement of the regulatory CD4+ T cells that produce high levels of interleukin-10 (IL-10) and low levels of IL-4 and IL-2 in the pathogenesis of rheumatoid arthritis (RA), we investigated whether the frequency of this type of CD4+ T cell subset in peripheral blood lymphocytes (PBL) or synovial lymphocyte infiltrates of patients with RA correlated with disease severity and histologic features in rheumatoid synovium,Methods. PBL and synovial lymphocyte infiltrates were isolated from peripheral blood samples and synovial tissues obtained from 25 patients with RA, Control specimens were obtained from 18 patients with osteoarthritis (OA) and 10 patients with traumatic injuries of the knee joint. CD4+ T cell subsets were categorized as Th1 (production of interferon-gamma [IFN gamma], butnot IL-4), Th2 (production of IL-4, but not IFN gamma), or CD4+ T cell subsets producing IL-10, IL-2, or IL-4, The percentages of these T helper subsets among PBL and among synovial infiltrating lymphocytes were determined by an intracellular staining assay with flow cytometric analysis. Results. The level of expression of CD4+ T cells producing IL-10 but not IL-2 and IL-4 in the peripheral blood and synovial tissue was significantly lower in RA patients than in OA patients and trauma patients. In RA patients, the frequency of this type of CD4+ T cell subset among synovial infiltrating CD4+ T cells was inversely correlated with the frequency of Th1 cells and the Th1/Th2 balance in synovial lymphocytes, serum C-reactive protein value, disease activity score, and the degree of synovial lining hyperplasiaand lymphocyte infiltration in rheumatoid synovium, There was a reciprocalrelationship between the frequency of Th1 cells and CD4+ T cells producingIL-10 but not IL-2 and IL-4 in the peripheral blood of RA patients. Conclusion. In RA, reduced expression of the CD4+ T cell subset producing IL-10 but not IL-2 and IL-4 may be responsible for the dominance of Th1 over Th2 cells at sites of inflamed synovium and in the peripheral blood, Decreases in this type of CD4+ T cell subset may induce the down-regulation of T cell tolerance and exacerbate the inflammatory process in RA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 10:34:00