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Titolo:
RECOGNITION OF PROMINENT VIRAL EPITOPES INDUCED BY IMMUNIZATION WITH HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 REGULATORY GENES
Autore:
HINKULA J; SVANHOLM C; SCHWARTZ S; LUNDHOLM P; BRYTTING M; ENGSTROM G; BENTHIN R; GLASER H; SUTTER G; KOHLEISEN B; ERFLE V; OKUDA K; WIGZELL H; WAHREN B;
Indirizzi:
SWEDISH INST INFECT DIS CONTROL,DEPT VIROL S-10521 STOCKHOLM SWEDEN KAROLINSKA INST,CTR MICROBIOL & TUMOR BIOL S-10521 STOCKHOLM SWEDEN FORSCHUNGSZENTRUM NEUBERG,INST MOL VIROL D-85764 OBERSCHLEISSHEIM GERMANY YOKOHAMA CITY UNIV,SCH MED,KANAZAWA KU YOKOHAMA KANAGAWA 232 JAPAN
Titolo Testata:
Journal of virology
fascicolo: 7, volume: 71, anno: 1997,
pagine: 5528 - 5539
SICI:
0022-538X(1997)71:7<5528:ROPVEI>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
B-CELL MEMORY; IMMUNE-RESPONSES; PLASMID DNA; INTRAMUSCULAR INJECTION; MONOCLONAL-ANTIBODIES; PROTECTIVE IMMUNITY; SYNTHETIC PEPTIDES; NEF; INFLUENZA; VACCINATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
49
Recensione:
Indirizzi per estratti:
Citazione:
J. Hinkula et al., "RECOGNITION OF PROMINENT VIRAL EPITOPES INDUCED BY IMMUNIZATION WITH HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 REGULATORY GENES", Journal of virology, 71(7), 1997, pp. 5528-5539

Abstract

Mice immunized with the regulatory genes nef, rev, and tat from humanimmunodeficiency virus type 1 developed both humoral and cellular immune responses to the gene products Nef, Rev, and Tat. This study demonstrates that it is feasible to induce immune reactions to all of theseregulatory gene products. Humoral responses were seen after DNA boosts, while potent T-cell proliferative responses were noted already after a single immunization. A Th1-directed immune response was demonstrated early after immunization. A 3- to 75-fold-stronger T-cell response mas seen in animals receiving DNA epidermally compared to that in animals receiving intramuscular injections. Nef, Rev, and Tat putative B- and T-cell epitopes were clearly mapped by using peptides derived fromthe regulatory proteins and were similar to those which are detected in human immunodeficiency virus infection. Although immunization by the Nef, Rev, and Tat proteins raised high immunoglobulin G titers in serum, the epitope spreading appeared broader after DNA immunization, The combination of all of these regulatory genes together with two genesfor structural proteins, the envelope and gag genes, demonstrated that a combined approach is feasible in that reactivities to all antigenspersisted or were even augmented. No interference between plasmids was noted.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 02:12:51