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Titolo:
INTERFERENCE OF CORONAVIRUS INFECTION BY EXPRESSION OF IMMUNOGLOBULIN-G (IGG) OR IGA VIRUS-NEUTRALIZING ANTIBODIES
Autore:
CASTILLA J; SOLA I; ENJUANES L;
Indirizzi:
CSIC,CTR NACL BIOTECNOL,DEPT CELL & MOL BIOL,CAMPUS UNIV AUTONOMA E-28049 MADRID SPAIN CSIC,CTR NACL BIOTECNOL,DEPT CELL & MOL BIOL E-28049 MADRID SPAIN
Titolo Testata:
Journal of virology
fascicolo: 7, volume: 71, anno: 1997,
pagine: 5251 - 5258
SICI:
0022-538X(1997)71:7<5251:IOCIBE>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSMISSIBLE GASTROENTERITIS CORONAVIRUS; SINGLE-CHAIN ANTIBODY; PORCINE RESPIRATORY CORONAVIRUS; INFLUENZA-VIRUS; INTRACELLULAR NEUTRALIZATION; ANTIGENIC SITES; E2 GLYCOPROTEIN; MUCOSAL DEFENSE; IMMUNE-RESPONSE; LYMPHOID-CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
50
Recensione:
Indirizzi per estratti:
Citazione:
J. Castilla et al., "INTERFERENCE OF CORONAVIRUS INFECTION BY EXPRESSION OF IMMUNOGLOBULIN-G (IGG) OR IGA VIRUS-NEUTRALIZING ANTIBODIES", Journal of virology, 71(7), 1997, pp. 5251-5258

Abstract

Immunoglobulin gene fragments encoding the variable modules of the heavy and light chains of a transmissible gastroenteritis coronavirus (TGEV)-neutralizing monoclonal antibody (MAb) have been cloned and sequenced, The selected MAb recognizes a highly conserved viral epitope anddoes not lead to the selection of neutralization escape mutants, The sequences of MAb 6A.C3 kappa and gamma 1 modules were identified as subgroup V and subgroup IIIC, respectively. The chimeric immunoglobulin genes encoding the variable modules from the murine MAb and constant modules of human gamma 1 and kappa chains were constructed by reverse transcriptase PCR, Chimeric immunoglobulins were stably or transiently expressed in murine myelomas or COS cells, respectively, The secreted recombinant antibodies had radioimmunoassay titers (i.e., the highest dilution giving a threefold increase over the background) higher than 10(3) and reduced the infectious virus more than 10(4)-fold, Recombinant dimeric immunoglobulin A (IgA) showed a 50-fold enhanced neutralization of TGEV relative to a recombinant monomeric IgG1 which contained the identical antigen binding site, Stably transformed epithelial celllines which expressed either recombinant IgG or IgA TGEV-neutralizingantibodies reduced virus production by > 10(5)-fold after infection with homologous virus, although a residual level of virus production (<10(2) PFU/ml) remained in less than 0.1% of the cells, This low-level persistent infection was shown not to be due to the selection of neutralization escape mutants, The implications of these findings for somatic gene therapy with recombinant antibodies are discussed.

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Documento generato il 11/07/20 alle ore 06:49:41